Microfluidic Paper-Based Analytical Device for Histidine Determination.
Amino acid
Aminoacyl-tRNA synthetase
Biosensing
Laminated paper-based analytical device
Microfluidics
Journal
Applied biochemistry and biotechnology
ISSN: 1559-0291
Titre abrégé: Appl Biochem Biotechnol
Pays: United States
ID NLM: 8208561
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
13
04
2020
accepted:
22
06
2020
pubmed:
28
6
2020
medline:
21
5
2021
entrez:
28
6
2020
Statut:
ppublish
Résumé
A laminated paper-based analytical device (LPAD) for histidine detection was fabricated from a chromatography filtration paper and laminate films. Histidine recognition was effected by histidyl-tRNA synthetase (HisRS), and its detection was signaled colorimetrically based on the molybdenum blue reaction. The analytical conditions and detectable concentration range of histidine were examined. The method provided selective quantification from 1 to 100 μM histidine. LPAD fabrication is considerably simple, involving only the craft-cutting of the chromatography filtration paper and laminate film, and is cost-effective.
Identifiants
pubmed: 32592084
doi: 10.1007/s12010-020-03365-z
pii: 10.1007/s12010-020-03365-z
doi:
Substances chimiques
Histidine
4QD397987E
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM