AXR1 affects DNA methylation independently of its role in regulating meiotic crossover localization.
Arabidopsis
/ genetics
Arabidopsis Proteins
/ genetics
Chromosome Pairing
Chromosome Segregation
Chromosomes, Plant
/ genetics
Crossing Over, Genetic
DNA Breaks, Double-Stranded
DNA Methylation
DNA Transposable Elements
/ genetics
Gene Knockout Techniques
Meiosis
/ genetics
Plants, Genetically Modified
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
16
07
2019
accepted:
29
05
2020
revised:
10
07
2020
pubmed:
1
7
2020
medline:
1
9
2020
entrez:
30
6
2020
Statut:
epublish
Résumé
Meiotic crossovers (COs) are important for reshuffling genetic information between homologous chromosomes and they are essential for their correct segregation. COs are unevenly distributed along chromosomes and the underlying mechanisms controlling CO localization are not well understood. We previously showed that meiotic COs are mis-localized in the absence of AXR1, an enzyme involved in the neddylation/rubylation protein modification pathway in Arabidopsis thaliana. Here, we report that in axr1-/-, male meiocytes show a strong defect in chromosome pairing whereas the formation of the telomere bouquet is not affected. COs are also redistributed towards subtelomeric chromosomal ends where they frequently form clusters, in contrast to large central regions depleted in recombination. The CO suppressed regions correlate with DNA hypermethylation of transposable elements (TEs) in the CHH context in axr1-/- meiocytes. Through examining somatic methylomes, we found axr1-/- affects DNA methylation in a plant, causing hypermethylation in all sequence contexts (CG, CHG and CHH) in TEs. Impairment of the main pathways involved in DNA methylation is epistatic over axr1-/- for DNA methylation in somatic cells but does not restore regular chromosome segregation during meiosis. Collectively, our findings reveal that the neddylation pathway not only regulates hormonal perception and CO distribution but is also, directly or indirectly, a major limiting pathway of TE DNA methylation in somatic cells.
Identifiants
pubmed: 32598340
doi: 10.1371/journal.pgen.1008894
pii: PGENETICS-D-19-01166
pmc: PMC7351236
doi:
Substances chimiques
AXR1 protein, Arabidopsis
0
Arabidopsis Proteins
0
DNA Transposable Elements
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1008894Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/J/000PR9773
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S009620/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P013511/1
Pays : United Kingdom
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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