Location of the Hydrophobic Surfactant Proteins, SP-B and SP-C, in Fluid-Phase Bilayers.
Journal
The journal of physical chemistry. B
ISSN: 1520-5207
Titre abrégé: J Phys Chem B
Pays: United States
ID NLM: 101157530
Informations de publication
Date de publication:
06 08 2020
06 08 2020
Historique:
pubmed:
1
7
2020
medline:
15
5
2021
entrez:
1
7
2020
Statut:
ppublish
Résumé
The hydrophobic surfactant proteins, SP-B and SP-C, promote rapid adsorption by the surfactant lipids to the surface of the liquid that lines the alveolar air sacks of the lungs. To gain insights into the mechanisms of their function, we used X-ray diffuse scattering (XDS) and molecular dynamics (MD) simulations to determine the location of SP-B and SP-C within phospholipid bilayers. Initial samples contained the surfactant lipids from extracted calf surfactant with increasing doses of the proteins. XDS located protein density near the phospholipid headgroup and in the hydrocarbon core, presumed to be SP-B and SP-C, respectively. Measurements on dioleoylphosphatidylcholine (DOPC) with the proteins produced similar results. MD simulations of the proteins with DOPC provided molecular detail and allowed direct comparison of the experimental and simulated results. Simulations used conformations of SP-B based on other members of the saposin-like family, which form either open or closed V-shaped structures. For SP-C, the amino acid sequence suggests a partial α-helix. Simulations fit best with measurements of XDS for closed SP-B, which occurred at the membrane surface, and SP-C oriented along the hydrophobic interior. Our results provide the most definitive evidence yet concerning the location and orientation of the hydrophobic surfactant proteins.
Identifiants
pubmed: 32600036
doi: 10.1021/acs.jpcb.0c03665
pmc: PMC8243356
mid: NIHMS1704312
doi:
Substances chimiques
Lipid Bilayers
0
Phospholipids
0
Pulmonary Surfactants
0
Surface-Active Agents
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6763-6774Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL060914
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL136734
Pays : United States
Organisme : NHLBI NIH HHS
ID : R56 HL130130
Pays : United States
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