Comparative transcriptomics revealed differential regulation of defense related genes in Brassica juncea leading to successful and unsuccessful infestation by aphid species.
Animals
Aphids
/ pathogenicity
Gene Expression
/ genetics
Gene Expression Profiling
/ methods
Gene Expression Regulation, Plant
/ genetics
Insecticides
Mustard Plant
/ genetics
Pest Control, Biological
/ methods
Plant Diseases
/ genetics
Sequence Analysis, RNA
/ methods
Transcriptome
/ genetics
Exome Sequencing
/ methods
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
29 06 2020
29 06 2020
Historique:
received:
27
11
2019
accepted:
18
05
2020
entrez:
1
7
2020
pubmed:
1
7
2020
medline:
16
1
2021
Statut:
epublish
Résumé
Productivity of Indian mustard (B. juncea), a major oil yielding crop in rapeseed-mustard group is heavily inflicted by mustard aphid, L. erysimi. Mustard aphid, a specialist aphid species on rapeseed-mustard crops, rapidly multiplies and colonizes the plants leading to successful infestation. In contrary, legume specific cowpea aphid, A. craccivora when released on B. juncea plants fails to build up population and thus remains unsuccessful in infestation. In the present study, differential host response of B. juncea to the two aphid species, one being successful insect-pest and the other being unsuccessful on it has been studied based on transcriptome analysis. Differential feeding efficiency of the two aphid species on mustard plants was evident from the amount of secreted honeydews. Leaf-transcriptomes of healthy and infested plants, treated with the two aphid species, were generated by RNA sequencing on Illumina platform and de novo assembly of the quality reads. A comparative assessment of the differentially expressed genes due to treatments revealed a large extent of overlaps as well as distinctness with respect to the set of genes and their direction of regulation. With respect to host-genes related to transcription factors, oxidative homeostasis, defense hormones and secondary metabolites, L. erysimi led to either suppression or limited activation of the transcript levels compared to A. craccivora. Further, a comprehensive view of the DEGs suggested more potential of successful insect-pests towards transcriptional reprogramming of the host. qRT-PCR based validation of randomly selected up- and down-regulated transcripts authenticated the transcriptome data.
Identifiants
pubmed: 32601289
doi: 10.1038/s41598-020-66217-0
pii: 10.1038/s41598-020-66217-0
pmc: PMC7324606
doi:
Substances chimiques
Insecticides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
10583Références
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