Identification and characterization of long noncoding RNAs and their association with acquisition of blood meal in Culex quinquefasciatus.
Culex quinquefasciatus
blood-feeding
long noncoding RNA
transcriptome
Journal
Insect science
ISSN: 1744-7917
Titre abrégé: Insect Sci
Pays: Australia
ID NLM: 101266965
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
revised:
20
06
2020
received:
30
01
2020
accepted:
22
06
2020
pubmed:
6
7
2020
medline:
10
8
2021
entrez:
5
7
2020
Statut:
ppublish
Résumé
The Southern house mosquito, Culex quinquefasciatus (Cx. quinquefasciatus) is an important vector that transmit multiple diseases including West Nile encephalitis, Japanese encephalitis, St. Louis encephalitis and lymphatic filariasis. Long noncoding RNAs (lncRNAs) involve in many biological processes such as development, infection, and virus-host interaction. However, there is no systematic identification and characterization of lncRNAs in Cx. quinquefasciatus. Here, we report the first lncRNA identification in Cx. quinquefasciatus. By using 31 public RNA-seq datasets, a total of 4763 novel lncRNA transcripts were identified, of which 3591, 569, and 603 were intergenic, intronic, and antisense respectively. Examination of genomic features revealed that Cx. quinquefasciatus shared similar characteristics with other species such as short in length, low GC content, low sequence conservation, and low coding potential. Furthermore, compared to protein-coding genes, Cx. quinquefasciatus lncRNAs had lower expression values, and tended to be expressed in temporally specific fashion. In addition, weighted correlation network and functional annotation analyses showed that lncRNAs may have roles in blood meal acquisition of adult female Cx. quinquefasciatus mosquitoes. This study presents the first systematic identification and analysis of Cx. quinquefasciatus lncRNAs and their association with blood feeding. Results generated from this study will facilitate future investigation on the function of Cx. quinquefasciatus lncRNAs.
Identifiants
pubmed: 32621332
doi: 10.1111/1744-7917.12847
doi:
Substances chimiques
RNA, Long Noncoding
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
917-928Subventions
Organisme : Universiti Sains Malaysia
ID : 1001/PBIOLOGI/811320
Informations de copyright
© 2020 Institute of Zoology, Chinese Academy of Sciences.
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