Transcriptome analysis of Clarias magur brain and gonads suggests neuro-endocrine inhibition of milt release from captive GnRH-induced males.
Animals
Brain
/ metabolism
Catfishes
/ genetics
Cholic Acid
/ biosynthesis
Female
Gonadotropin-Releasing Hormone
/ pharmacology
Male
Neurophysins
/ metabolism
Ovary
/ metabolism
RNA-Seq
Reproduction
/ genetics
Semen
Testis
/ metabolism
Testosterone
/ analogs & derivatives
Transcriptome
gamma-Aminobutyric Acid
/ metabolism
Freshwater catfishes
Induced breeding
NGS
Next generation sequencing
RNASeq
Reproductive genes
Journal
Genomics
ISSN: 1089-8646
Titre abrégé: Genomics
Pays: United States
ID NLM: 8800135
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
04
05
2020
revised:
18
06
2020
accepted:
03
07
2020
pubmed:
11
7
2020
medline:
16
9
2021
entrez:
11
7
2020
Statut:
ppublish
Résumé
Transcriptome analysis of Clarias magur brain and gonads at preparatory, mature, 6 and 16 h post-GnRH injection (hpi) stages yielded 9.5 GB data with 39,738 contigs. Sequences of 45 reproductive genes were identified for the first time in C. magur along with unique and differentially expressed genes. The expression of 20 genes was validated by qRT-PCR. Upregulation of Cyp11A1, Cyp17A1 and FTZF1 genes in the 16hpi testis accompanied by the 17β-HSD3 expression indicates testosterone (T) synthesis in response to LH surge, while reduced expression of CYP11B1 suggests a high T: 11-KT ratio. It is evident by the gene expression analysis that the inhibitory neurotransmitter GABA, altered T: 11-KT, increased testicular bile acids, and oxytocin-like neuropeptide in the male brain, appear to be involved in arresting the pulsatile motion of testicular smooth muscles. The work generates important leads for an effective induced breeding strategy for silurid catfish.
Identifiants
pubmed: 32650102
pii: S0888-7543(20)30511-5
doi: 10.1016/j.ygeno.2020.07.012
pii:
doi:
Substances chimiques
Neurophysins
0
Gonadotropin-Releasing Hormone
33515-09-2
Testosterone
3XMK78S47O
gamma-Aminobutyric Acid
56-12-2
Cholic Acid
G1JO7801AE
11-ketotestosterone
KF38W1A85U
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4041-4052Informations de copyright
Copyright © 2020. Published by Elsevier Inc.