High abundance of genus Prevotella is associated with dysregulation of IFN-I and T cell response in HIV-1-infected patients.
ADP-ribosyl Cyclase 1
Adult
CD4-Positive T-Lymphocytes
/ immunology
CD8-Positive T-Lymphocytes
/ immunology
Case-Control Studies
Dysbiosis
/ immunology
HIV Infections
/ immunology
HIV-1
HLA-DR Antigens
Humans
Interferon-beta
/ immunology
Lymphocyte Activation
Male
Middle Aged
Myxovirus Resistance Proteins
/ immunology
Pilot Projects
Prevotella
/ isolation & purification
Receptor, Interferon alpha-beta
/ immunology
Journal
AIDS (London, England)
ISSN: 1473-5571
Titre abrégé: AIDS
Pays: England
ID NLM: 8710219
Informations de publication
Date de publication:
01 08 2020
01 08 2020
Historique:
entrez:
18
7
2020
pubmed:
18
7
2020
medline:
20
2
2021
Statut:
ppublish
Résumé
HIV-1-associated dysbiosis is most commonly characterized by overall decreased diversity, with abundance of the genus Prevotella, recently related to inflammatory responses. A pilot study including 10 antiretroviral therapy-treated HIV-1-infected men and 50 uninfected controls was performed to identify the main gut dysbiosis determinants (e.g. Prevotella enrichment), that may affect mucosal antiviral defenses and T cell immunity in HIV-1-infected individuals. 16rRNA gene sequencing was applied to the HIV-1-infected individuals' fecal microbiota and compared with controls. Measurements of CD4 and CD8 T cell activation [CD38, human leukocyte antigen (HLA)-DR, CD38 HLA-DR] and frequencies of Th17, obtained from lamina propria lymphocytes isolated from five different intestinal sites, were performed by flow cytometry. IFNβ, IFNAR1 and MxA gene expression level was evaluated by real-time PCR in lamina propria lymphocytes. Nonparametric t tests were used for statistical analysis. HIV-1-infected men had a significant fecal microbial communities' imbalance, including different levels of genera Faecalibacterium, Prevotella, Alistipes and Bacteroides, compared with controls. Notably, Prevotella abundance positively correlated with frequencies of CD4 T cells expressing CD38 or HLA-DR and coexpressing CD38 and HLA-DR (P < 0.05 for all these measures). The same trend was observed for the activated CD8 T cells. Moreover, Prevotella levels were inversely correlated with IFN-I genes (P < 0.05 for IFNβ, IFNAR1 and MxA genes) and the frequencies of Th17 cells (P < 0.05). By contrast, no statistically significant correlations were observed for the remaining bacterial genera. Our findings suggest that Prevotella enrichment might affect gut mucosal IFN-I pathways and T cell response in HIV-1-infected patients, thus contributing to immune dysfunction.
Identifiants
pubmed: 32675560
doi: 10.1097/QAD.0000000000002574
pii: 00002030-202008010-00005
doi:
Substances chimiques
HLA-DR Antigens
0
IFNAR1 protein, human
0
MX1 protein, human
0
Myxovirus Resistance Proteins
0
Receptor, Interferon alpha-beta
156986-95-7
Interferon-beta
77238-31-4
ADP-ribosyl Cyclase 1
EC 3.2.2.6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1467-1473Références
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