Ethylene glycol and glycolic acid production by wild-type Escherichia coli.
E. coli
ethylene glycol
glycolic acid
xylonic acid
xylose
Journal
Biotechnology and applied biochemistry
ISSN: 1470-8744
Titre abrégé: Biotechnol Appl Biochem
Pays: United States
ID NLM: 8609465
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
pubmed:
20
7
2020
medline:
30
9
2021
entrez:
20
7
2020
Statut:
ppublish
Résumé
Ethylene glycol and glycolic acid are bulk chemicals with a broad range of applications. The ethylene glycol and glycolic acid biosynthesis pathways have been produced by microorganisms and used as a biological route for their production. Unlike the methods that use xylose or glucose as carbon sources, xylonic acid was used as a carbon source to produce ethylene glycol and glycolic acid in this study. Amounts of 4.2 g/L of ethylene glycol and 0.7 g/L of glycolic acid were produced by a wild-type Escherichia coli W3110 within 10 H of cultivation with a substrate conversion ratio of 0.5 mol/mol. Furthermore, E. coli strains that produce solely ethylene glycol or glycolic acid were constructed. 10.3 g/L of glycolic acid was produced by E. coli ΔyqhD+aldA, and the achieved conversion ratio was 0.56 mol/mol. Similarly, the E. coli ΔaldA+yqhD produced 8.0 g/L of ethylene glycol with a conversion ratio of 0.71 mol/mol. Ethylene glycol and glycolic acid production by E. coli on xylonic acid as a carbon source provides new information on the biosynthesis pathway of these products and opens a novel way of biomass utilization.
Substances chimiques
Escherichia coli Proteins
0
Glycolates
0
glycolic acid
0WT12SX38S
Aldehyde Reductase
EC 1.1.1.21
YqhD protein, E coli
EC 1.1.1.21
Aldehyde Oxidoreductases
EC 1.2.-
Ethylene Glycol
FC72KVT52F
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
744-755Subventions
Organisme : National Key R&D Program of China
ID : 2017YFE0112700
Organisme : Royal Society-Newton Advanced Fellowship
ID : NAF∖R2∖180721
Organisme : Natural Science Foundation of Shanghai
ID : 19ZR1463600
Organisme : National Natural Science Foundation of China
ID : 21576279
Informations de copyright
© 2020 International Union of Biochemistry and Molecular Biology, Inc.
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