Combinatorial synthetic pathway fine-tuning and cofactor regeneration for metabolic engineering of Escherichia coli significantly improve production of D-glucaric acid.

Biosynthetic Pathways Biotechnology Escherichia coli / enzymology Glucaric Acid / metabolism Inositol Oxygenase / metabolism Metabolic Engineering
Microbial fermentation Platform chemical RBS optimization Self-sufficient Systematic metabolic engineering

Journal

New biotechnology
ISSN: 1876-4347
Titre abrégé: N Biotechnol
Pays: Netherlands
ID NLM: 101465345

Informations de publication

Date de publication:
25 Nov 2020
Historique:
received: 08 11 2019
revised: 18 03 2020
accepted: 28 03 2020
pubmed: 22 7 2020
medline: 15 12 2020
entrez: 22 7 2020
Statut: ppublish

Résumé

D-glucaric acid (GA) has been identified as among promising biotechnological alternatives to oil-based chemicals. GA and its derivatives are widely used in food additives, dietary supplements, drugs, detergents, corrosion inhibitors and biodegradable materials. The increasing availability of a GA market is improving the cost-effectiveness and efficiency of various biosynthetic pathways. In this study, an engineered Escherichia coli strain GA10 was constructed by systematic metabolic engineering. This involved redirecting metabolic flux into the GA biosynthetic pathways, blocking the conversion pathways of d-glucuronic acid (GlcA) and GA into by-products, introducing an in situ NAD

Identifiants

DOI: 10.1016/j.nbt.2020.03.004 PMID: 32693027
pubmed: 32693027
pii: S1871-6784(20)30152-7
doi: 10.1016/j.nbt.2020.03.004
pii:
doi:

Substances chimiques

Inositol Oxygenase EC 1.13.99.1
Glucaric Acid QLZ991V4A2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

51-58

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Auteurs

Hui-Hui Su (HH)

Laboratory of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou 510640, China.

Fei Peng (F)

Laboratory of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou 510640, China.

Xiao-Yang Ou (XY)

Laboratory of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou 510640, China.

Ying-Jie Zeng (YJ)

Laboratory of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou 510640, China.

Min-Hua Zong (MH)

Laboratory of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou 510640, China.

Wen-Yong Lou (WY)

Laboratory of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou 510640, China. Electronic address: wylou@scut.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Métabolisme › Voies et réseaux métaboliques › Voies de biosynthèse › Combinatorial synthetic pathway fine-tuning...
questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Biotechnologie › Combinatorial synthetic pathway fine-tuning...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Combinatorial synthetic pathway fine-tuning...
questionsmedicales.fr › Glucides › Oses acides › Acide D-glucarique › Combinatorial synthetic pathway fine-tuning...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Métalloprotéines › Protéines de liaison au fer › Protéines à fer non hème › Inositol oxygenase › Combinatorial synthetic pathway fine-tuning...
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Ingénierie cellulaire › Génie métabolique › Combinatorial synthetic pathway fine-tuning...