Majority sensing in synthetic microbial consortia.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
21 07 2020
21 07 2020
Historique:
received:
10
02
2020
accepted:
25
06
2020
entrez:
23
7
2020
pubmed:
23
7
2020
medline:
10
9
2020
Statut:
epublish
Résumé
As synthetic biocircuits become more complex, distributing computations within multi-strain microbial consortia becomes increasingly beneficial. However, designing distributed circuits that respond predictably to variation in consortium composition remains a challenge. Here we develop a two-strain gene circuit that senses and responds to which strain is in the majority. This involves a co-repressive system in which each strain produces a signaling molecule that signals the other strain to down-regulate production of its own, orthogonal signaling molecule. This co-repressive consortium links gene expression to ratio of the strains rather than population size. Further, we control the cross-over point for majority via external induction. We elucidate the mechanisms driving these dynamics by developing a mathematical model that captures consortia response as strain fractions and external induction are varied. These results show that simple gene circuits can be used within multicellular synthetic systems to sense and respond to the state of the population.
Identifiants
pubmed: 32694598
doi: 10.1038/s41467-020-17475-z
pii: 10.1038/s41467-020-17475-z
pmc: PMC7374166
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
3659Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM117138
Pays : United States
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