A Click Chemistry Approach to Developing Molecularly Targeted DNA Scissors.


Journal

Chemistry (Weinheim an der Bergstrasse, Germany)
ISSN: 1521-3765
Titre abrégé: Chemistry
Pays: Germany
ID NLM: 9513783

Informations de publication

Date de publication:
15 Dec 2020
Historique:
received: 13 06 2020
revised: 23 07 2020
pubmed: 25 7 2020
medline: 20 2 2021
entrez: 25 7 2020
Statut: ppublish

Résumé

Nucleic acid click chemistry was used to prepare a family of chemically modified triplex forming oligonucleotides (TFOs) for application as a new gene-targeted technology. Azide-bearing phenanthrene ligands-designed to promote triplex stability and copper binding-were 'clicked' to alkyne-modified parallel TFOs. Using this approach, a library of TFO hybrids was prepared and shown to effectively target purine-rich genetic elements in vitro. Several of the hybrids provide significant stabilisation toward melting in parallel triplexes (>20 °C) and DNA damage can be triggered upon copper binding in the presence of added reductant. Therefore, the TFO and 'clicked' ligands work synergistically to provide sequence-selectivity to the copper cutting unit which, in turn, confers high stabilisation to the DNA triplex. To extend the boundaries of this hybrid system further, a click chemistry-based di-copper binding ligand was developed to accommodate designer ancillary ligands such as DPQ and DPPZ. When this ligand was inserted into a TFO, a dramatic improvement in targeted oxidative cleavage is afforded.

Identifiants

pubmed: 32706904
doi: 10.1002/chem.202002860
doi:

Substances chimiques

Ligands 0
Oligonucleotides 0
Copper 789U1901C5
DNA 9007-49-2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

16782-16792

Subventions

Organisme : H2020 Marie Skłodowska-Curie Actions
ID : H2020-MSCA-ITN-2014-642023
Organisme : Deutsche Forschungsgemeinschaft
ID : 201269156 (SFB1032)
Organisme : Carlsbergfondet
ID : CF15-0675
Organisme : Science Foundation Ireland
ID : 15/CDA/3648
Pays : Ireland
Organisme : Science Foundation Ireland
ID : 17/RC-PhD/3480
Pays : Ireland
Organisme : Science Foundation Ireland
ID : 18/TIDA/6111
Pays : Ireland

Informations de copyright

© 2020 Wiley-VCH GmbH.

Références

G. Biffi, D. Tannahill, J. McCafferty, S. Balasubramanian, Nat. Chem. 2013, 5, 182-186.
M. Zeraati, D. B. Langley, P. Schofield, A. L. Moye, R. Rouet, W. E. Hughes, T. M. Bryan, M. E. Dinger, D. Christ, Nat. Chem. 2018, 10, 631-637.
A. L. Brogden, N. H. Hopcroft, M. Searcey, C. J. Cardin, Angew. Chem. Int. Ed. 2007, 46, 3850-3854;
Angew. Chem. 2007, 119, 3924-3928.
K. R. Fox, T. Brown, D. A. Rusling, DNA-targeting Molecules as Therapeutic Agents, The Royal Society of Chemistry (Ed.: M. J. Waring), 2018, pp. 1-32.
K. Hoogsteen, Acta Crystallogr. 1959, 12, 822-823.
 
L. G. Carrascosa, S. Gomez-Montes, A. Avino, A. Nadal, M. Pla, R. Eritja, L. M. Lechuga, Nucleic Acids Res. 2012, 40, e56;
Q. Wu, S. S. Gaddis, M. C. MacLeod, E. F. Walborg, H. D. Thames, J. DiGiovanni, K. M. Vasquez, Mol. Carcinogenesis 2007, 46, 15-23.
 
H. E. Moser, P. B. Dervan, Science 1987, 238, 645-650;
G. Wang, M. M. Seidman, P. M. Glazer, Science 1996, 271, 802-805.
Y. Hu, A. Cecconello, A. Idili, F. Ricci, I. Willner, Angew. Chem. Int. Ed. 2017, 56, 15210-15233;
Angew. Chem. 2017, 129, 15410-15434.
H. Okamura, Y. Taniguchi, S. Sasaki, Angew. Chem. Int. Ed. 2016, 55, 12445-12449;
Angew. Chem. 2016, 128, 12633-12637.
 
S. Geny, P. M. Moreno, T. Krzywkowski, O. Gissberg, N. K. Andersen, A. J. Isse, A. M. El-Madani, C. Lou, Y. V. Pabon, B. A. Anderson, E. M. Zaghloul, R. Zain, P. J. Hrdlicka, P. T. Jorgensen, M. Nilsson, K. E. Lundin, E. B. Pedersen, J. Wengel, C. I. Smith, Nucleic Acids Res. 2016, 44, 2007-2019;
S. K. Singh, A. A. Koshkin, J. Wengel, P. Nielsen, Chem. Commun. 1998, 455-456.
P. E. Nielsen, M. Egholm, R. H. Berg, O. Buchardt, Science 1991, 254, 1497-1500.
J. S. Sun, J. C. Francois, T. Montenay-Garestier, T. Saison-Behmoaras, V. Roig, N. T. Thuong, C. Helene, Proc. Natl. Acad. Sci. USA 1989, 86, 9198-9202.
 
M. Paramasivam, S. Cogoi, V. V. Filichev, N. Bomholt, E. B. Pedersen, L. E. Xodo, Nucleic Acids Res. 2008, 36, 3494-3507;
V. V. Filichev, E. B. Pedersen, J. Am. Chem. Soc. 2005, 127, 14849-14858.
 
E. B. Pedersen, A. M. A. Osman, D. Globisch, M. Paramasivam, S. Cogoi, N. Bomholt, P. T. Jorgensen, L. E. Xodo, V. V. Filichev, Nucleic Acids Symposium Series 2008, 52, 37-38;
A. M. A. Osman, P. T. Jorgensen, N. Bomholt, E. B. Pedersen, Bioorg. Med. Chem. 2008, 16, 9937-9947.
 
S. Walsh, A. H. El-Sagheer, T. Brown, Chem. Sci. 2018, 9, 7681-7687;
P. Klimkowski, S. De Ornellas, D. Singleton, A. H. El-Sagheer, T. Brown, Org. Biomol. Chem. 2019, 17, 5943-5950.
 
D. S. Sigman, T. W. Bruice, A. Mazumder, C. L. Sutton, Acc. Chem. Res. 1993, 26, 98-104;
J. C. Francois, T. Saison-Behmoaras, C. Barbier, M. Chassignol, N. T. Thuong, C. Helene, Proc. Natl. Acad. Sci. USA 1989, 86, 9702-9706.
 
M. Pitié, B. Sudres, B. Meunier, Chem. Commun. 1998, 2597-2598;
M. Pitié, B. Donnadieu, B. Meunier, Inorg. Chem. 1998, 37, 3486-3489.
 
M. Pitié, B. Meunier, Bioconjugate Chem. 1998, 9, 604-611;
M. Pitié, J. D. Van Horn, D. Brion, C. J. Burrows, B. Meunier, Bioconjugate Chem. 2000, 11, 892-900;
R. Pitie, C. J. Burrows, B. Meunier, Nucleic Acids Res. 2000, 28, 4856-4864;
B. C. Bales, T. Kodama, Y. N. Weledji, M. Pitie, B. Meunier, M. M. Greenberg, Nucleic Acids Res. 2005, 33, 5371-5379;
J.-C. Francois, M. Faria, D. Perrin, C. Giovannangeli, Artificial Nucleases, Ed.: M. A. Zenkova, Springer Berlin Heidelberg, Berlin, Heidelberg, 2004, pp. 223-242.
A. Panattoni, A. H. El-Sagheer, T. Brown, A. Kellett, M. Hocek, ChemBioChem 2020, 21, 991-1000.
 
J. Gierlich, G. A. Burley, P. M. Gramlich, D. M. Hammond, T. Carell, Org. Lett. 2006, 8, 3639-3642;
P. M. Gramlich, S. Warncke, J. Gierlich, T. Carell, Angew. Chem. Int. Ed. 2008, 47, 3442-3444;
Angew. Chem. 2008, 120, 3491-3493;
A. H. El-Sagheer, T. Brown, Chem. Soc. Rev. 2010, 39, 1388-1405;
G. Gasser, A. Pinto, S. Neumann, A. M. Sosniak, M. Seitz, K. Merz, R. Heumann, N. Metzler-Nolte, Dalton Trans. 2012, 41, 2304-2313.
 
C. Slator, Z. Molphy, V. McKee, C. Long, T. Brown, A. Kellett, Nucleic Acids Res. 2018, 46, 2733-2750;
Z. Molphy, D. Montagner, S. S. Bhat, C. Slator, C. Long, A. Erxleben, A. Kellett, Nucleic Acids Res. 2018, 46, 9918-9931.
 
Z. Molphy, A. Prisecaru, C. Slator, N. Barron, M. McCann, J. Colleran, D. Chandran, N. Gathergood, A. Kellett, Inorg. Chem. 2014, 53, 5392-5404;
A. Kellett, Z. Molphy, C. Slator, V. McKee, N. P. Farrell, Chem. Soc. Rev. 2019, 48, 971-988.
 
M. Levine, Y. Wang, S. J. Padayatty, J. Morrow, Proc. Natl. Acad. Sci. USA 2001, 98, 9842-9846;
M. Levine, C. Conry-Cantilena, Y. Wang, R. W. Welch, P. W. Washko, K. R. Dhariwal, J. B. Park, A. Lazarev, J. F. Graumlich, J. King, L. R. Cantilena, Proc. Natl. Acad. Sci. USA 1996, 93, 3704-3709;
Q. Chen, M. G. Espey, A. Y. Sun, J. H. Lee, M. C. Krishna, E. Shacter, P. L. Choyke, C. Pooput, K. L. Kirk, G. R. Buettner, M. Levine, Proc. Natl. Acad. Sci. USA 2007, 104, 8749-8754.
 
M. Murtola, M. Wenska, R. Strömberg, J. Am. Chem. Soc. 2010, 132, 8984-8990;
O. Luige, M. Murtola, A. Ghidini, R. Strömberg, Molecules 2019, 24, 672.
 
K. J. Humphreys, K. D. Karlin, S. E. Rokita, J. Am. Chem. Soc. 2002, 124, 8055-8066;
T. Ito, S. Thyagarajan, K. D. Karlin, S. E. Rokita, Chem. Commun. 2005, 38, 4812-4814.
 
N. Z. Fantoni, Z. Molphy, S. O'Carroll, G. Menounou, G. Mitrikas, M. G. Krokidis, C. Chatgilialoglu, J. Colleran, A. Banasiak, M. Clynes, S. Roche, S. Kelly, V. McKee, A. Kellett, Chem. Eur. J. 2020, https://doi.org/10.1002/chem.202001996;
N. Z. Fantoni, Z. Molphy, C. Slator, G. Menounou, G. Toniolo, G. Mitrikas, V. McKee, C. Chatgilialoglu, A. Kellett, Chem. Eur. J. 2019, 25, 221-237;
N. Zuin Fantoni, B. McGorman, Z. Molphy, D. Singleton, S. Walsh, A. H. El-Sagheer, V. McKee, T. Brown, A. Kellett, ChemBioChem, 2020, https://doi.org/10.1002/cbic.202000408.
A. Pasternak, J. Wengel, Org. Biomol. Chem. 2011, 9, 3591-3597.
 
C. N. Birts, A. P. Sanzone, A. H. El-Sagheer, J. P. Blaydes, T. Brown, A. Tavassoli, Angew. Chem. Int. Ed. 2014, 53, 2362-2365;
Angew. Chem. 2014, 126, 2394-2397;
A. P. Sanzone, A. H. El-Sagheer, T. Brown, A. Tavassoli, Nucleic Acids Res. 2012, 40, 10567-10575;
A. H. El-Sagheer, A. P. Sanzone, R. Gao, A. Tavassoli, T. Brown, Proc. Natl. Acad. Sci. USA 2011, 108, 11338-11343.
P. Kumar, A. H. El-Sagheer, L. Truong, T. Brown, Chem. Commun. 2017, 53, 8910-8913.
J. Ohata, F. Vohidov, A. Aliyan, K. Huang, A. A. Martí, Z. T. Ball, Chem. Commun. 2015, 51, 15192-15195.
G. Gou, R. Ren, S. Li, S. Guo, Z. Dong, M. Xie, J. Ma, New J. Chem. 2013, 37, 3861-3864.
H.-X. Wang, K.-G. Zhou, Y.-L. Xie, J. Zeng, N.-N. Chai, J. Li, H.-L. Zhang, Chem. Commun. 2011, 47, 5747-5749.
 
J. E. Jones, R. L. Jenkins, R. S. Hicks, A. J. Hallett, S. J. A. Pope, Dalton Trans. 2012, 41, 10372-10381;
G.-J. Lin, G.-B. Jiang, Y.-Y. Xie, H.-L. Huang, Z.-H. Liang, Y.-J. Liu, J. Biol. Inorg. Chem. 2013, 18, 873-882.
S. Ji, H. Guo, X. Yuan, X. Li, H. Ding, P. Gao, C. Zhao, W. Wu, W. Wu, J. Zhao, Org. Lett. 2010, 12, 2876-2879.
K. Duskova, L. Gude, M.-S. Arias-Pérez, Tetrahedron 2014, 70, 1071-1076.
K. F. Jennings, J. Chem. Soc. 1957, 1172-1175.
F. Cheng, M. Ren, C. He, H. Yin, Inorg. Chim. Acta 2016, 450, 170-175.
G. M. Sheldrick, Acta Crystallogr. Sect. A 2015, 71, 3-8.
G. M. Sheldrick, Acta Crystallogr. Sect. C 2015, 71, 3-8.
M. Su, A. Kirchner, S. Stazzoni, M. Müller, M. Wagner, A. Schröder, T. Carell, Angew. Chem. Int. Ed. 2016, 55, 11797-11800;
Angew. Chem. 2016, 128, 11974-11978.

Auteurs

Teresa Lauria (T)

School of Chemical Sciences and National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin, 9, Ireland.

Creina Slator (C)

School of Chemical Sciences and National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin, 9, Ireland.

Vickie McKee (V)

School of Chemical Sciences and National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin, 9, Ireland.
Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, 5230, Odense M, Denmark.

Markus Müller (M)

Department of Chemistry, Ludwig-Maximilians-Universität, Butenandtstrasse 5-13, 81377, Munich, Germany.

Samuele Stazzoni (S)

Department of Chemistry, Ludwig-Maximilians-Universität, Butenandtstrasse 5-13, 81377, Munich, Germany.

Antony L Crisp (AL)

Department of Chemistry, Ludwig-Maximilians-Universität, Butenandtstrasse 5-13, 81377, Munich, Germany.

Thomas Carell (T)

Department of Chemistry, Ludwig-Maximilians-Universität, Butenandtstrasse 5-13, 81377, Munich, Germany.

Andrew Kellett (A)

School of Chemical Sciences and National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin, 9, Ireland.
CÚRAM, Centre for Research in Medical Devices, Dublin City University, Glasnevin, Dublin, 9, Ireland.

Articles similaires

A molecular mechanism for bright color variation in parrots.

Roberto Arbore, Soraia Barbosa, Jindich Brejcha et al.
1.00
Animals Feathers Pigmentation Parrots Aldehyde Dehydrogenase
Receptor, Cannabinoid, CB1 Ligands Molecular Dynamics Simulation Protein Binding Thermodynamics
DNA Methylation Humans DNA Animals Machine Learning
DNA Glycosylases Nucleosomes Humans 8-Hydroxy-2'-Deoxyguanosine DNA Repair

Classifications MeSH