The transcription factor WRKY22 is required during cryo-stress acclimation in Arabidopsis shoot tips.
Abiotic stress
cryoprotectant
shoot tip
stomatal closure
transcription factor
transcriptomics
ultra-low temperature
Journal
Journal of experimental botany
ISSN: 1460-2431
Titre abrégé: J Exp Bot
Pays: England
ID NLM: 9882906
Informations de publication
Date de publication:
06 08 2020
06 08 2020
Historique:
received:
17
10
2019
accepted:
24
07
2020
pubmed:
28
7
2020
medline:
15
5
2021
entrez:
26
7
2020
Statut:
ppublish
Résumé
Storage of meristematic tissue at ultra-low temperatures offers a mean to maintain valuable genetic resources from vegetatively reproduced plants. To reveal the biology underlying cryo-stress, shoot tips of the model plant Arabidopsis thaliana were subjected to a standard preservation procedure. A transcriptomic approach was taken to describe the subsequent cellular events which occurred. The cryoprotectant treatment induced the changes in the transcript levels of genes associated with RNA processing and primary metabolism. Explants of a mutant lacking a functional copy of the transcription factor WRKY22 were compromised for recovery. A number of putative downstream targets of WRKY22 were identified, some related to phytohormone-mediated defense, to the osmotic stress response, and to development. There were also alterations in the abundance of transcript produced by genes encoding photosynthesis-related proteins. The wrky22 mutant plants developed an open stomata phenotype in response to their exposure to the cryoprotectant solution. WRKY22 probably regulates a transcriptional network during cryo-stress, linking the explant's defense and osmotic stress responses to changes in its primary metabolism. A model is proposed linking WRKY53 and WRKY70 downstream of the action of WRKY22.
Identifiants
pubmed: 32710609
pii: 5876280
doi: 10.1093/jxb/eraa224
pmc: PMC7475261
doi:
Substances chimiques
Arabidopsis Proteins
0
Transcription Factors
0
WRKY22 protein, Arabidopsis
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4993-5009Informations de copyright
© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.
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