Use of a combination of the MD simulations and NMR spectroscopy to determine the regulatory mechanism of pulsed electric field (PEF) targeting at C-terminal histidine of VNAVLH.
Antioxidant activity
MD simulation
NMR spectroscopy
Pine nut peptides
Pulsed electric field
Secondary structure
Journal
Food chemistry
ISSN: 1873-7072
Titre abrégé: Food Chem
Pays: England
ID NLM: 7702639
Informations de publication
Date de publication:
01 Jan 2021
01 Jan 2021
Historique:
received:
17
02
2020
revised:
30
06
2020
accepted:
08
07
2020
pubmed:
28
7
2020
medline:
3
11
2020
entrez:
26
7
2020
Statut:
ppublish
Résumé
In this study, the targeted regulatory mechanism of pulsed electric field (PEF) was explored for antioxidant activity improvement in four peptides, RGAVIH, RGAVLH, VNAVIH, and VNAVLH, of the pine nut (Pinus koraiensis Sieb. et Zucc). The VNAVLH peptide exhibited the best antioxidant activity and the β-sheet content decreased to a minimum value at 40 kV/cm. Moreover, the chemical shifts of hydrogen atoms of 2-H Asn and 6-H His shifted to a higher magnetic field. The connectivity between N
Identifiants
pubmed: 32711267
pii: S0308-8146(20)31416-3
doi: 10.1016/j.foodchem.2020.127554
pii:
doi:
Substances chimiques
Antioxidants
0
Peptides
0
Histidine
4QD397987E
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
127554Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.