Changes in antimicrobial resistance and molecular attributes of Salmonellae causing enteric fever in Kolkata, India, 2014-2018.
Adolescent
Anti-Bacterial Agents
/ pharmacology
Child
Child, Preschool
Cluster Analysis
Clustered Regularly Interspaced Short Palindromic Repeats
Drug Resistance, Multiple, Bacterial
Female
Genetic Variation
Humans
India
/ epidemiology
Infant
Male
Microbial Sensitivity Tests
Multilocus Sequence Typing
Salmonella paratyphi A
/ drug effects
Salmonella typhi
/ drug effects
Typhoid Fever
/ epidemiology
Antimicrobial resistance
CRISPR
MLVA
PFGE
S. Paratyphi A
S. Typhi
Journal
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
ISSN: 1567-7257
Titre abrégé: Infect Genet Evol
Pays: Netherlands
ID NLM: 101084138
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
03
02
2020
revised:
22
07
2020
accepted:
23
07
2020
pubmed:
1
8
2020
medline:
14
10
2021
entrez:
1
8
2020
Statut:
ppublish
Résumé
Globally, enteric fever caused by Salmonella Typhi (S. Typhi, ST) and S. Paratyphi A (SPA) remain one of the major diseases of public health importance. In this study, a total of 457 (380 ST, 77 SPA) blood isolates were collected from three tertiary care hospitals in Kolkata during 2014-18. Additionally, 66 (3.4%) ST and 5 (0.25%) SPA were recovered from blood culture of 1962 patients attending OPD of one pediatric hospital during 2016-18. The study isolates were tested for antimicrobial resistance (AMR) profiles; AMR genes; molecular sub-types by PFGE, MLVA and CRISPR. Among the total 446 ST and 82 SPA isolates, fluoroquinolone (FQ) resistance was very common in both serovars. Ciprofloxacin resistance of 24.9% and 9.8% & ofloxacin resistance of 20.9% and 87.8% were found in ST and SPA respectively. Majority (>70%) of the isolates showed decreased susceptibility to ciprofloxacin (DCS). A single point mutation in gyrA gene (S83F) was responsible for causing DCS in 37.5% (n = 42/112) ST and 63% (n = 46/73) SPA isolates. Multidrug resistance (MDR) was found only in 3.4% ST isolates and encoded the genes bla
Identifiants
pubmed: 32736039
pii: S1567-1348(20)30309-9
doi: 10.1016/j.meegid.2020.104478
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
104478Informations de copyright
Copyright © 2020. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.