Heterologous Expression of the Barley (Hordeum vulgare L.) Xantha-f, -g and -h Genes that Encode Magnesium Chelatase Subunits.
Barley
Chlorophyll biosynthesis
Magnesium chelatase
Mg-chelatase
Protoporphyrin
Xantha
Journal
The protein journal
ISSN: 1875-8355
Titre abrégé: Protein J
Pays: Netherlands
ID NLM: 101212092
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
pubmed:
2
8
2020
medline:
4
2
2021
entrez:
2
8
2020
Statut:
ppublish
Résumé
Biosynthesis of chlorophyll involves several enzymatic reactions of which many are shared with the heme biosynthesis pathway. Magnesium chelatase is the first specific enzyme in the chlorophyll pathway. It catalyzes the formation of Mg-protoporphyrin IX from the insertion of Mg
Identifiants
pubmed: 32737834
doi: 10.1007/s10930-020-09913-0
pii: 10.1007/s10930-020-09913-0
pmc: PMC7704502
doi:
Substances chimiques
Plant Proteins
0
Recombinant Proteins
0
Lyases
EC 4.-
magnesium chelatase
EC 4.99.1-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
554-562Références
Willows RD, Hansson M (2003) Mechanism, structure, and regulation of magnesium chelatase. In: Kadish KM, Smith KM, Guilard R (eds) The porphyrin handbook: chlorophylls and bilins: biosynthesis, synthesis and degradation, vol 13. Elsevier, San Diego, pp 1–47
Kannangara CG, von Wettstein D (2010) Magnesium chelatase. In: Rebeiz CA, et al. (eds) The chloroplast. Advances in photosynthesis and respiration, vol 31. Springer, Dordrecht, pp 79–88
Hansson M, Lundqvist J, Sirijovski N, Al-Karadaghi S (2014) Magnesium chelatase: The molecular motor of chlorophyll biosynthesis. In: Ferreira GC, Kadish KM, Smith KM, Guilard R (eds) Handbook of porphyrin science with applications to chemistry, physics, materials science, engineering, biology and medicine (Vol 28, pp 41–84). World Scientific Publishing Co, Singapore
Willows RD (2019) The Mg branch of chlorophyll synthesis: biosynthesis of chlorophyll a from protoporphyrin IX. Adv Bot Res 90:141–182
von Wettstein D, Kahn A, Nielsen OF, Gough S (1974) Genetic regulation of chlorophyll synthesis analyzed with mutants in barley. Science 184:800–802
pubmed: 17783474
Marrs B (1981) Mobilization of the genes for photosynthesis from Rhodopseudomonas capsulata by a promiscuous plasmid. J Bacteriol 146:1003–1012
pubmed: 6787008
pmcid: 216954
Bollivar DW, Suzuki JY, Beatty JT et al (1994) Directed mutational analysis of bacteriochlorophyll a biosynthesis in Rhodobacter capsulatus. J Mol Biol 237:622–640
pubmed: 8158642
Gibson LC, Willows RD, Kannangara CG, von Wettstein D, Hunter CN (1995) Magnesium-protoporphyrin chelatase of Rhodobacter sphaeroides: reconstitution of activity by combining the products of the bchH, -I, and -D genes expressed in Escherichia coli. Proc Natl Acad Sci USA 92:1941–1944
pubmed: 7892204
Willows RD, Gibson LCD, Kanangara CG, Hunter CN, von Wettstein D (1996) Three separate proteins constitute the magnesium chelatase of Rhodobacter sphaeroides. Eur J Biochem 235:438–443
pubmed: 8631364
Jensen PE, Gibson LCD, Henningsen KW, Hunter CN (1996) Expression of the chlI, chlD, and chlH genes from the cyanobacterium Synechocystis PCC6803 in Escherichia coli and demonstration that the three cognate proteins are required for magnesium-protoporphyrin chelatase activity. J Biol Chem 271:16662–16667
pubmed: 8663186
Farmer DA, Brindley AA, Hitchcock A, Jackson PJ, Johnson B, Dickman MJ, Hunter CN, Reid JD, Adams NBP (2019) The ChlD subunit links the motor and porphyrin binding subunits of magnesium chelatase. Biochem J 476:1875–1887
pubmed: 31164400
pmcid: 6604950
Gorchein A (1973) Control of magnesium–protoporphyrin chelatase activity in Rhodopseudomonas spheroides. Role of light, oxygen, and electron and energy transfer. Biochem J 134:833–845
pubmed: 4202754
pmcid: 1177891
Castelfranco PA, Weinstein JD, Schwarcz S, Pardo AD, Wezelman BE (1979) The Mg insertion step in chlorophyll biosynthesis. Arch Biochem Biophys 192:592–598
pubmed: 434841
Lundqvist J, Elmlund H, Peterson Wulff R, Berglund L, Elmlund D, Emanuelsson C, Hebert H, Willows RD, Hansson M, Lindahl M, Al-Karadaghi S (2010) ATP-induced conformational dynamics in the AAA+ motor unit of magnesium chelatase. Structure 18:354–365
pubmed: 20223218
Sawicki A, Willows RD (2008) Kinetic analyses of the magnesium chelatase provide insights into the mechanism, structure, and formation of the complex. J Biol Chem 283:31294–31302
pubmed: 18790730
Axelsson E, Lundqvist J, Sawicki A, Nilsson S, Schröder I, Al-Karadaghi S, Willows RD, Hansson M (2006) Recessiveness and dominance in barley mutants deficient in Mg-chelatase subunit D, an AAA protein involved in chlorophyll biosynthesis. Plant Cell 18:3606–3616
pubmed: 17158606
pmcid: 1785401
Mandel M, Higa A (1970) Calcium-dependent bacteriophage DNA infection. J Mol Biol 53:159–162
Sirijovski N, Olsson U, Lundqvist J, Al-Karadaghi S, Willows RD, Hansson M (2006) ATPase activity associated with the magnesium chelatase H-subunit of the chlorophyll biosynthetic pathway is an artefact. Biochem J 400:477–484
pubmed: 16928192
pmcid: 1698598
Willows RD, Beale SI (1998) Heterologous expression of the Rhodobacter capsulatus BchI, -D, and -H genes that encode magnesium chelatase subunits and characterization of the reconstituted enzyme. J Biol Chem 273:34206–34213
pubmed: 9852082
Emanuelsson O, Nielsen H, von Heijne G (1999) ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites. Protein Sci 8:978–984
pubmed: 10338008
pmcid: 2144330
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549
pubmed: 29722887
pmcid: 5967553
Kannangara CG, Vothknecht UC, Hansson M, von Wettstein D (1997) Magnesium chelatase: association with ribosomes and mutant complementation studies identify barley subunit Xantha-G as a functional counterpart of Rhodobacter subunit BchD. Mol Gen Genet 254:85–92
pubmed: 9108294
Tanaka R, Tanaka A (2007) Tetrapyrrole biosynthesis in higher plants. Annu Rev Plant Biol 58:321–346
pubmed: 17227226
Adams NB, Brindley AA, Hunter CN, Reid JD (2016) The catalytic power of magnesium chelatase: a benchmark for the AAA(+) ATPases. FEBS Lett 590:1687–1693
pubmed: 27176620
pmcid: 4982103
Schubert HL, Raux E, Wilson KS, Warren MJ (1999) Common chelatase design in the branched tetrapyrrole pathways of heme and anaerobic cobalamin synthesis. Biochem 38:10660–10669
Henningsen KW, Boynton JE, von Wettstein D (1993) Mutants at xantha and albina loci in relation to chloroplast biogenesis in barley (Hordeum vulgare L.). The Royal Danish Academy of Sciences and Letters 42:1–349
Jensen PE, Petersen BL, Stummann BM, Henningsen KW, Willows RD, Vothknecht UC, Kannangara CG, von Wettstein D (1996) Structural genes for Mg-chelatase subunits in barley: Xantha-f, -g and -h. Mol Gen Genet 250:383–394
pubmed: 8602155
Hansson A, Kannangara CG, von Wettstein D, Hansson M (1999) Molecular basis for semidominance of missense mutations in the XANTHA-H (42-kDa) subunit of magnesium chelatase. Proc Natl Acad Sci USA 96:1744–1749
pubmed: 9990095
Hansson A, Willows RD, Roberts TH, Hansson M (2002) Three semidominant barley mutants with single amino acid substitutions in the smallest magnesium chelatase subunit form defective AAA+ hexamers. Proc Natl Acad Sci USA 99:13944–13949
pubmed: 12357035
Braumann I, Stein N, Hansson M (2014) Reduced chlorophyll biosynthesis in heterozygous barley magnesium chelatase mutants. Plant Physiol Biochem 78:10–14
pubmed: 24607574
Olsson U, Sirijovski N, Hansson M (2004) Characterization of eight barley xantha-f mutants deficient in magnesium chelatase. Plant Physiol Biochem 42:557–564
pubmed: 15246070
Sirijovski N, Mamedov F, Olsson U, Styring S, Hansson M (2007) Rhodobacter capsulatus magnesium chelatase subunit BchH contains an oxygen sensitive iron-sulfur cluster. Arch Microbiol 188:599–608
pubmed: 17639347
Fodje MN, Hansson A, Hansson M, Olsen JG, Gough S, Willows RD, Al-Karadaghi S (2001) Interplay between an AAA module and an integrin I domain may regulate the function of magnesium chelatase. J Mol Biol 311:111–122
pubmed: 11469861
Willows RD, Hansson A, Birch D, Al-Karadaghi S, Hansson M (2004) EM single particle analysis of the ATP-dependent BchI complex of magnesium chelatase: an AAA(+) hexamer. J Struct Biol 146:227–233
pubmed: 15037253
Sirijovski N, Lundqvist J, Rosenbäck M, Elmlund H, Al-Karadaghi S, Willows RD, Hansson M (2008) Substrate-binding model of the chlorophyll biosynthetic magnesium chelatase BchH subunit. J Biol Chem 283:11652–11660
pubmed: 18263581
Elmlund H, Lundqvist J, Al-Karadaghi S, Hansson M, Hebert H, Lindahl M (2008) A new cryo-EM single-particle ab initio reconstruction method visualizes secondary structure elements in an ATP-fueled AAA+ motor. J Mol Biol 375:934–947
pubmed: 18068723
Lundqvist J, Elmlund D, Heldt D, Deery E, Söderberg C, Hansson M, Warren M, Al-Karadaghi S (2009) The AAA(+) motor complex of subunits CobS and CobT of cobaltochelatase visualized by single particle electron microscopy. J Struct Biol 167:227–234
pubmed: 19545636
Qian P, Marklew CJ, Viney J, Davison PA, Brindley AA, Söderberg C, Al-Karadaghi S, Bullough PA, Grossmann JG, Hunter CN (2012) Structure of the cyanobacterial magnesium chelatase H subunit determined by single particle reconstruction and small-angle x-ray scattering. J Biol Chem 287:4946–4956
pubmed: 22179610
Adams NBP, Marklew CJ, Qian P, Brindley AA, Davison PA, Bullough PA, Hunter CN (2014) Structural and functional consequences of removing the N-terminal domain from the magnesium chelatase ChlH subunit of Thermosynechococcus elongatus. Biochem J 464:315–322
pubmed: 25471602
pmcid: 4255732
Reid JD, Siebert CA, Bullough PA, Hunter CN (2003) The ATPase activity of the ChlI subunit of magnesium chelatase and formation of a heptameric AAA_ ring. Biochemistry 42:6912–6920
pubmed: 12779346
Gao Y-S, Wang Y-L, Wang X, Liu L (2019) Hexameric structure of the ATPase motor subunit of magnesium chelatase in chlorophyll biosynthesis. Protein Sci 29:1026–1032
Chen X, Pu H, Fang Y, Wang X, Zhao S, Lin Y, Zhang M, Dai H-E, Gong W, Liu L (2015) Crystal structure of the catalytic subunit of magnesium chelatase. Nature Plants 1:15125
pubmed: 27250678