Genomic analyses of a livestock pest, the New World screwworm, find potential targets for genetic control programs.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
04 08 2020
04 08 2020
Historique:
received:
30
12
2019
accepted:
14
07
2020
entrez:
6
8
2020
pubmed:
6
8
2020
medline:
22
6
2021
Statut:
epublish
Résumé
The New World Screwworm fly, Cochliomyia hominivorax, is a major pest of livestock in South America and Caribbean. However, few genomic resources have been available for this species. A genome of 534 Mb was assembled from long read PacBio DNA sequencing of DNA from a highly inbred strain. Analysis of molecular evolution identified 40 genes that are likely under positive selection. Developmental RNA-seq analysis identified specific genes associated with each stage. We identify and analyze the expression of genes that are likely important for host-seeking behavior (chemosensory), development of larvae in open wounds in warm-blooded animals (heat shock protein, immune response) and for building transgenic strains for genetic control programs including gene drive (sex determination, germline). This study will underpin future experiments aimed at understanding the parasitic lifestyle of the screwworm fly and greatly facilitate future development of strains for efficient systems for genetic control of screwworm.
Identifiants
pubmed: 32753684
doi: 10.1038/s42003-020-01152-4
pii: 10.1038/s42003-020-01152-4
pmc: PMC7403345
doi:
Banques de données
Dryad
['10.5061/dryad.x69p8czg2']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
424Références
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