Immunomodulatory and antitumour bioactive labdane diterpenoids from Leonurus japonicus.
Animals
Antineoplastic Agents, Phytogenic
/ isolation & purification
Carcinoma, Lewis Lung
/ drug therapy
Cell Proliferation
/ drug effects
Cytokines
/ metabolism
Diterpenes
/ isolation & purification
HT29 Cells
Hep G2 Cells
Humans
Immunologic Factors
/ isolation & purification
Inflammation Mediators
/ metabolism
Leonurus
/ chemistry
Leukocytes, Mononuclear
/ drug effects
MCF-7 Cells
Macrophages
/ drug effects
Male
Mice
Mice, Inbred C57BL
Plant Extracts
/ isolation & purification
RAW 264.7 Cells
T-Lymphocyte Subsets
/ drug effects
Tumor Burden
/ drug effects
Leonurus japonicus
cytotoxicity
heteronone
labdane diterpenoids
leojapone
lewis lung cancer
Journal
The Journal of pharmacy and pharmacology
ISSN: 2042-7158
Titre abrégé: J Pharm Pharmacol
Pays: England
ID NLM: 0376363
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
09
03
2020
accepted:
04
07
2020
pubmed:
7
8
2020
medline:
5
8
2021
entrez:
7
8
2020
Statut:
ppublish
Résumé
Two labdane diterpenoids, leojapone B and heteronone B, were isolated from Leonurus japonicus Houtt., and their biological activity were evaluated in this study. Human and mouse cancer cells, human peripheral blood mononuclear cells (PBMCs) and mouse macrophages (RAW264.7 cells) were used to evaluate the activity of leojapone B and heteronone B, while the in vivo effects of leojapone B were further examined in Lewis Lung Cancer tumour-bearing mice. In vitro studies showed that leojapone B selectively inhibited the proliferation of lung cancer cells, and both leojapone B and heteronone B inhibited the production of pro-inflammatory cytokines in activated PBMCs. In tumour-bearing mice model, lung tumours were reduced in size in mice treated with intraperitoneal injections of leojapone B at 20 and 30 mg/kg for 14 days. The population ratio of CD4 The inhibitory effects of leojapone B in mouse lung tumours were demonstrated for the first time in this study. The immunomodulatory activity of heteronone B were also demonstrated. Our findings indicated that both leojapone B and heteronone B may act as active components in L. japonicus.
Substances chimiques
Antineoplastic Agents, Phytogenic
0
Cytokines
0
Diterpenes
0
Immunologic Factors
0
Inflammation Mediators
0
Plant Extracts
0
heteronone B
0
labdane
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1657-1665Subventions
Organisme : the Program for Changjiang Scholars and Innovative Research Team in University
ID : IRT_17R94
Organisme : National Natural Science Foundation of China
ID : 21762048
Organisme : National Natural Science Foundation of China
ID : 81422046
Organisme : National Natural Science Foundation of China
ID : 81860615
Organisme : Applied Basic Research Foundation of Yunnan Province
ID : 2018FA048
Organisme : Applied Basic Research Foundation of Yunnan Province
ID : 2018FY001
Informations de copyright
© 2020 Royal Pharmaceutical Society.
Références
Xiong L et al. Sesquiterpenoids from the herb of Leonurus japonicus. Molecules 2013; 18: 5051-5071.
Ye M et al. Leonurusoleanolides E-J, minor spirocyclic triterpenoids from Leonurus japonicus fruits. J Nat Prod 2014; 77: 178-182.
Wu H et al. Isoleojaponin, a new halimane diterpene isolated from Leonurus japonicus. Molecules 2015; 20: 839-845.
Liu J et al. Alkaloids and flavonoid glycosides from the aerial parts of Leonurus japonicus and their opposite effects on uterine smooth muscle. Phytochemistry 2017; 145: 128-136.
Zhang Y et al. Rare syringyl acylated flavonol glycosides from the aerial parts of Leonurus japonicus Houtt. Molecules 2013; 18: 2967-2977.
Li Y et al. Hepatoprotective glycosides from Leonurus japonicus Houtt. Carbohydr Res 2012; 348: 42-46.
Morita H et al. Cycloleonuripeptides E and F, cyclic nonapeptides from Leonurus heterophyllus. J Nat Prod 2006; 69: 839-841.
He YL et al. Angiogenic effect of motherwort (Leonurus japonicus) alkaloids and toxicity of motherwort essential oil on zebrafish embryos. Fitoterapia 2018; 128: 36-42.
Xiong L et al. Bis-spirolabdane diterpenoids from Leonurus japonicus and their anti-platelet aggregative activity. Fitoterapia 2015; 100: 1-6.
Zhong WM et al. Three minor new compounds from the aerial parts of Leonurus japonicus. Chin Chem Lett 2015; 26: 1000-1003.
Khan S et al. Anti-inflammatory mechanism of 15,16-epoxy-3α-hydroxylabda-8,13(16),14-trien-7-one via inhibition of LPS-induced multicellular signaling pathways. J Nat Prod 2012; 75: 67-71.
Hu YM et al. Two new labdane diterpenoids from aerial parts of Leonurus japonicus and their anti-inflammatory activity. Nat Prod Res 2018; 9: 1-8.
Moon HI. Three diterpenes from Leonurus japonicus Houtt protect primary cultured rat cortical cells from glutamate-induced toxicity. Phytother Res 2010; 24: 1256-1259.
Nguyen LTT et al. Labdane and norlabdane diterpenoids from the aerial parts of Leonurus japonicus. Phytochem Lett 2017; 22: 174-178.
Wu HK et al. New diterpenoids isolated from Leonurus japonicus and their acetylcholinesterase inhibitory activity. Chin J Nat Med 2017; 15: 860-864.
Jiang MH et al. A new labdane-type diterpenoid from Leonurus japonicus. J Asian Nat Prod Res 2019; 21: 627-632.
Peng Y et al. Novel labdane diterpenoids from the aerial parts of Leonurus japonicus. Phytochem Lett 2017; 20: 45-48.
Zhang X, Peng GP. Two labdane diterpenes from Leonurus heterophyllus. Chin Chem Lett 2006; 17: 1321-1324.
Yue GGL et al. Anti-angiogenic and immunomodulatory activities of a labdane diterpene from Leonurus heterophyllus. World J Tradit Chin Med 2016; 2: 81.
Luo KW et al. Anti-tumor and anti-osteolysis effects of the metronomic use of zoledronic acid in primary and metastatic breast cancer mouse models. Cancer Lett 2013; 339: 42-48.
Yue GGL et al. New potential beneficial effects of actein, a triterpene glycoside isolated from Cimicifuga species, in breast cancer treatment. Sci Rep 2016; 6: 35263.
Yue GGL et al. Immunomodulatory activities of Ganoderma sinense polysaccharides in human immune cells. Nutr Cancer 2013; 65: 765-774.
Yue GGL et al. Screening for anti-inflammatory and bronchorelaxant activities of 12 commonly used Chinese herbal medicines. Phytother Res 2012; 26: 915-925.
Miao LL et al. Leonurus japonicus (Chinese motherwort), an excellent traditional medicine for obstetrical and gynecological diseases: A comprehensive overview. Biomed Pharmacother 2019; 117: 109060.
Shang X et al. Leonurus japonicus Houtt.: ethnopharmacology, phytochemistry and pharmacology of an important traditional Chinese medicine. J Ethnopharmacol 2014; 152: 14-32.
Tao J et al. Cytotoxicity of Chinese motherwort (YiMuCao) aqueous ethanol extract is non-apoptotic and estrogen receptor independent on human breast cancer cells. J Ethnopharmacol 2009; 122: 234-239.
Xiao CJ et al. Localisation of two bioactive labdane diterpenoids in the peltate glandular trichomes of Leonurus japonicus by laser microdissection coupled with UPLC-MS/MS. Phytochem Anal. 2017; 28: 404-409.
Peng W et al. Two new oleanane derivatives from the fruits of Leonurus japonicus and their cytotoxic activities. J Nat Med 2019; 73: 252-256.
Li H et al. New labdane diterpenoids from Leonurus japonicus and their anti-inflammatory activity. Phytochem Lett 2014; 10: 313-317.
Bronte V, Pittet MJ. The spleen in local and systemic regulation of immunity. Immunity 2013; 39: 806-818.
Li C et al. Foxp3 overexpression decreases sensitivity to chemotherapy in mouse Lewis lung cancer cells. Mol Med Rep 2012; 6: 977-982.
Szczepanski MJ et al. Increased frequency and suppression by regulatory T cells in patients with acute myelogenous leukemia. Clin Cancer Res 2009; 15: 3325-3332.
Ozao-Choy J et al. The novel role of tyrosine kinase inhibitor in the reversal of immune suppression and modulation of tumor microenvironment for immune-based cancer therapies. Cancer Res 2009; 69: 2514-2522.