Simultaneous visualisation of the complete sets of telomeres from the MmeI generated terminal restriction fragments in yeasts.
Saccharomyces cerevisiae
Saccharomyces paradoxus
Southern blotting
X element
X-element-only telomere
Y′ element
Y′-element-containing telomere
restriction endonuclease MmeI
telomere
telomere length
terminal restriction fragment
yeast
Journal
Yeast (Chichester, England)
ISSN: 1097-0061
Titre abrégé: Yeast
Pays: England
ID NLM: 8607637
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
12
05
2020
revised:
02
08
2020
accepted:
03
08
2020
pubmed:
11
8
2020
medline:
4
9
2021
entrez:
11
8
2020
Statut:
ppublish
Résumé
Telomere length is measured using Southern blotting of the chromosomal terminal restriction fragments (TRFs) released by endonuclease digestion in cells from yeast to human. In the budding yeast Saccharomyces cerevisiae, XhoI or PstI is applied to cut the subtelomere Y' element and release TRFs from the 17 subtelomeres. However, telomeres from other 15 X-element-only subtelomeres are omitted from analysis. Here, we report a method for measuring all 32 telomeres in S. cerevisiae using the endonuclease MmeI. Based on analyses of the endonuclease cleavage sites, we found that the TRFs generated by MmeI displayed two distinguishable bands in the sizes of ~500 and ~700 bp comprising telomeres (300 bp) and subtelomeres (200-400 bp). The modified MmeI-restricted TRF (mTRF) method recapitulated telomere shortening and lengthening caused by deficiencies of YKu and Rif1 respectively in S. cerevisiae. Furthermore, we found that mTRF was also applicable to telomere length analysis in S. paradoxus strains. These results demonstrate a useful tool for simultaneous detection of telomeres from all chromosomal ends with both X-element-only and Y'-element subtelomeres in S. cerevisiae species.
Substances chimiques
Repressor Proteins
0
Saccharomyces cerevisiae Proteins
0
Telomere-Binding Proteins
0
RIF1 protein, S cerevisiae
146589-82-4
endodeoxyribonuclease MmeI
EC 3.1.21.-
Deoxyribonucleases, Type II Site-Specific
EC 3.1.21.4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
585-595Informations de copyright
© 2020 John Wiley & Sons, Ltd.
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