Identification of transcription factors controlling floral morphology in wild Petunia species with contrasting pollination syndromes.
Petunia
ACME
MYB
RNAseq
biomechanics
floral morphology
growth analysis
speciation genes
style
transcription factors
transcriptomics
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
04
10
2019
accepted:
15
07
2020
pubmed:
12
8
2020
medline:
22
6
2021
entrez:
12
8
2020
Statut:
ppublish
Résumé
Adaptation to different pollinators is an important driver of speciation in the angiosperms. Genetic approaches such as QTL mapping have been successfully used to identify the underlying speciation genes. However, these methods are often limited by widespread suppression of recombination due to divergence between species. While the mutations that caused the interspecific differences in floral color and scent have been elucidated in a variety of plant genera, the genes that are responsible for morphological differences remain mostly unknown. Differences in floral organ length determine the pollination efficiency of hawkmoths and hummingbirds, and therefore the genes that control these differences are potential speciation genes. Identifying such genes is challenging, especially in non-model species and when studying complex traits for which little prior genetic and biochemical knowledge is available. Here we combine transcriptomics with detailed growth analysis to identify candidate transcription factors underlying interspecific variation in the styles of Petunia flowers. Starting from a set of 2284 genes, stepwise filtering for expression in styles, differential expression between species, correlation with growth-related traits, allele-specific expression in interspecific hybrids, and/or high-impact polymorphisms resulted in a set of 43 candidate speciation genes. Validation by virus-induced gene silencing identified two MYB transcription factors, EOBI and EOBII, that were previously shown to regulate floral scent emission, a trait associated with pollination by hawkmoths.
Identifiants
pubmed: 32780443
doi: 10.1111/tpj.14962
pmc: PMC7693086
doi:
Substances chimiques
Plant Proteins
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
289-301Subventions
Organisme : Swiss National Science Foundation
ID : 31003A_182340
Pays : Switzerland
Organisme : European Research Council
ID : 741354
Pays : International
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2020 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.
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