Expression of a
2A
Malassezia
bicistronic
fluorescent
multicistronic
tagging
transformation
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2020
2020
Historique:
received:
15
04
2020
accepted:
15
06
2020
entrez:
15
8
2020
pubmed:
15
8
2020
medline:
22
6
2021
Statut:
epublish
Résumé
The use of fluorescent proteins allows a multitude of approaches from live imaging and fixed cells to labeling of whole organisms, making it a foundation of diverse experiments. Tagging a protein of interest or specific cell type allows visualization and studies of cell localization, cellular dynamics, physiology, and structural characteristics. In specific instances fluorescent fusion proteins may not be properly functional as a result of structural changes that hinder protein function, or when overexpressed may be cytotoxic and disrupt normal biological processes. In our study, we describe application of a bicistronic vector incorporating a Picornavirus 2A peptide sequence between a
Identifiants
pubmed: 32793513
doi: 10.3389/fcimb.2020.00367
pmc: PMC7387403
doi:
Substances chimiques
Codon
0
Luminescent Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
367Subventions
Organisme : NIAID NIH HHS
ID : R01 AI039115
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI050113
Pays : United States
Informations de copyright
Copyright © 2020 Goh, Ianiri, Heitman and Dawson.
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