NsiR3, a nitrogen stress-inducible small RNA, regulates proline oxidase expression in the cyanobacterium Nostoc sp. PCC 7120.
5' Untranslated Regions
Ammonium Compounds
/ chemistry
Bacterial Proteins
/ chemistry
Base Sequence
Binding Sites
Cloning, Molecular
Escherichia coli
/ genetics
Gene Expression
Gene Expression Regulation, Bacterial
Genetic Vectors
/ chemistry
Membrane Proteins
/ chemistry
Nitrogen
/ chemistry
Nitrogen Fixation
/ genetics
Nostoc
/ genetics
Nucleic Acid Conformation
Protein Binding
Protein Interaction Domains and Motifs
Protein Processing, Post-Translational
RNA, Bacterial
/ chemistry
RNA, Small Untranslated
/ chemistry
Recombinant Fusion Proteins
/ chemistry
Sequence Alignment
Sequence Homology, Nucleic Acid
Transcription Factors
/ genetics
Transcription, Genetic
NtcA
PutA
heterocyst
post-transcriptional regulation
regulatory RNA
Journal
The FEBS journal
ISSN: 1742-4658
Titre abrégé: FEBS J
Pays: England
ID NLM: 101229646
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
14
05
2020
revised:
17
07
2020
accepted:
10
08
2020
pubmed:
18
8
2020
medline:
21
7
2021
entrez:
18
8
2020
Statut:
ppublish
Résumé
NsiR3 (nitrogen stress-inducible RNA 3) is a small noncoding RNA strongly conserved in heterocyst-forming cyanobacteria. In Nostoc sp. PCC 7120, transcription of NsiR3 is induced by nitrogen starvation and depends on the global nitrogen regulator NtcA. A conserved NtcA-binding site is centered around position -42.5 with respect to the transcription start site of NsiR3 homologs, and NtcA binds in vitro to a DNA fragment containing this sequence. In the absence of combined nitrogen, NsiR3 expression is induced in all cells along the Nostoc filament but much more strongly in heterocysts, differentiated cells devoted to nitrogen fixation. Co-expression analysis of transcriptomic data obtained from microarrays hybridized with RNA obtained from Nostoc wild-type or mutant strains grown in the presence of ammonium or in the absence of combined nitrogen revealed that the expression profile of gene putA (proline oxidase) correlates negatively with that of NsiR3. Using a heterologous system in Escherichia coli, we show that NsiR3 binds to the 5'-UTR of putA mRNA, resulting in reduced expression of a reporter gene. Overexpression of NsiR3 in Nostoc resulted in strong reduction of putA mRNA accumulation, further supporting the negative regulation of putA by NsiR3. The higher expression of NsiR3 in heterocysts versus vegetative cells of the N
Substances chimiques
5' Untranslated Regions
0
Ammonium Compounds
0
Bacterial Proteins
0
Membrane Proteins
0
PutA protein, Bacteria
0
RNA, Bacterial
0
RNA, Small Untranslated
0
Recombinant Fusion Proteins
0
Transcription Factors
0
Nitrogen
N762921K75
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1614-1629Informations de copyright
© 2020 Federation of European Biochemical Societies.
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