The crystal structure of protein-transporting chaperone BCP1 from Saccharomyces cerevisiae.

Binding Sites / physiology Crystallography, X-Ray / methods Nuclear Proteins / chemistry Protein Conformation, beta-Strand / physiology Protein Structure, Secondary / physiology Ribosomal Proteins / chemistry Ribosomes / metabolism Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / chemistry
BCP1 Chaperone Crystal structure GNAT PF13862

Journal

Journal of structural biology
ISSN: 1095-8657
Titre abrégé: J Struct Biol
Pays: United States
ID NLM: 9011206

Informations de publication

Date de publication:
01 10 2020
Historique:
received: 26 05 2020
revised: 04 08 2020
accepted: 12 08 2020
pubmed: 18 8 2020
medline: 14 10 2021
entrez: 18 8 2020
Statut: ppublish

Résumé

BCP1 is a protein enriched in the nucleus that is required for Mss4 nuclear export and identified as the chaperone of ribosomal protein Rpl23 in Saccharomyces cerevisiae. According to sequence homology, BCP1 is related to the mammalian BRCA2-interacting protein BCCIP and belongs to the BCIP protein family (PF13862) in the Pfam database. However, the BCIP family has no discernible similarity to proteins with known structure. Here, we report the crystal structure of BCP1, presenting an α/β fold in which the central antiparallel β-sheet is flanked by helices. Protein structural classification revealed that BCP1 has similarity to the GNAT superfamily but no conserved substrate-binding residues. Further modeling and protein-protein docking work provide a plausible model to explain the interaction between BCP1 and Rpl23. Our structural analysis presents the first structure of BCIP family and provides a foundation for understanding the molecular basis of BCP1 as a chaperone of Rpl23 for ribosome biosynthesis.

Identifiants

DOI: 10.1016/j.jsb.2020.107605 PMID: 32805410
pubmed: 32805410
pii: S1047-8477(20)30178-7
doi: 10.1016/j.jsb.2020.107605
pii:
doi:

Substances chimiques

Bcp1 protein, S cerevisiae 0
Nuclear Proteins 0
Ribosomal Proteins 0
Saccharomyces cerevisiae Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

107605

Informations de copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

Auteurs

Meng-Hsuan Lin (MH)

Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan; Genome and Systems Biology Degree Program, National Taiwan University and Academia Sinica, Taipei 10617, Taiwan.

Po-Chih Kuo (PC)

Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan.

Yi-Chih Chiu (YC)

Genome and Systems Biology Degree Program, National Taiwan University and Academia Sinica, Taipei 10617, Taiwan.

Yu-Yung Chang (YY)

Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan.

Sheng-Chia Chen (SC)

Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan.

Chun-Hua Hsu (CH)

Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan; Genome and Systems Biology Degree Program, National Taiwan University and Academia Sinica, Taipei 10617, Taiwan; Institute of Biochemical Sciences, National Taiwan University, Taipei 10617, Taiwan. Electronic address: andyhsu@ntu.edu.tw.

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Classifications MeSH

questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Sites de fixation › The crystal structure of protein-transporting...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Cristallographie aux rayons X › The crystal structure of protein-transporting...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines nucléaires › The crystal structure of protein-transporting...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure secondaire des protéines › Structure en brin bêta › The crystal structure of protein-transporting...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure secondaire des protéines › The crystal structure of protein-transporting...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines ribosomiques › The crystal structure of protein-transporting...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Ribosomes › The crystal structure of protein-transporting...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Saccharomyces › Saccharomyces cerevisiae › The crystal structure of protein-transporting...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines fongiques › Protéines de Saccharomyces cerevisiae › The crystal structure of protein-transporting...