Biochemical activity of magnesium ions on human osteoblast migration.
Alkaline Phosphatase
/ metabolism
Cell Line
Cell Movement
/ drug effects
Epithelial-Mesenchymal Transition
/ physiology
Gene Expression Regulation
/ drug effects
Humans
Magnesium Chloride
/ pharmacology
Microscopy, Fluorescence
Osteoblasts
/ cytology
Sequence Analysis, RNA
Snail Family Transcription Factors
/ metabolism
Zonula Occludens-1 Protein
/ metabolism
ALP activity
Cell migration
Magnesium
Osteoblast
SNAI2
ZO-1
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
22 10 2020
22 10 2020
Historique:
received:
14
06
2020
accepted:
12
07
2020
pubmed:
21
8
2020
medline:
20
3
2021
entrez:
21
8
2020
Statut:
ppublish
Résumé
Magnesium is well known as a biodegradable biomaterial that has been reported to promote bone remodeling in several studies; however, the underlying biological mechanism remains unclear. In the present study, the role of magnesium ions in the migration of U-2 OS cells, which are osteoblast-like cell lines, was investigated. Magnesium treatment did not significantly alter the global transcriptome of U-2 OS cells, but increased the protein expression level of SNAI2, an epithelial-mesenchymal transition (EMT) marker. In addition, it was confirmed that the junctional site localization of Zona-occludens 1 (ZO-1), a representative tight junction protein, was destroyed by magnesium treatment; furthermore, it was determined that cytoplasmic localization increased, and alkaline phosphatase (ALP) activity increased. The obtained results on the mechanism by which magnesium is involved in osteoblast migration, which is important for fracture healing, will contribute to the understanding of the bone-formation process in patients with osteoporosis and musculoskeletal injury.
Identifiants
pubmed: 32814632
pii: S0006-291X(20)31447-9
doi: 10.1016/j.bbrc.2020.07.057
pii:
doi:
Substances chimiques
SNAI2 protein, human
0
Snail Family Transcription Factors
0
TJP1 protein, human
0
Zonula Occludens-1 Protein
0
Magnesium Chloride
02F3473H9O
Alkaline Phosphatase
EC 3.1.3.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
588-594Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.