Conditional Recruitment to a DNA-Bound CRISPR-Cas Complex Using a Colocalization-Dependent Protein Switch.
CRISPR−Cas
Co-LOCKR
genetic circuit
protein switch
Journal
ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075
Informations de publication
Date de publication:
18 09 2020
18 09 2020
Historique:
pubmed:
21
8
2020
medline:
11
8
2021
entrez:
21
8
2020
Statut:
ppublish
Résumé
To spatially control biochemical functions at specific sites within a genome, we have engineered a synthetic switch that activates when bound to its DNA target site. The system uses two CRISPR-Cas complexes to colocalize components of a
Identifiants
pubmed: 32816470
doi: 10.1021/acssynbio.0c00012
pmc: PMC7976376
mid: NIHMS1680053
doi:
Substances chimiques
RNA, Guide
0
Recombinant Fusion Proteins
0
DNA
9007-49-2
CRISPR-Associated Protein 9
EC 3.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2316-2323Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM124773
Pays : United States
Organisme : NHGRI NIH HHS
ID : T32 HG000035
Pays : United States
Références
Nat Commun. 2017 Jul 13;8:15993
pubmed: 28703221
Nat Biotechnol. 2016 Sep 08;34(9):933-941
pubmed: 27606440
Mol Cell. 2014 May 22;54(4):698-710
pubmed: 24837679
Cell. 2015 Jan 15;160(1-2):339-50
pubmed: 25533786
Nat Commun. 2017 Nov 22;8(1):1688
pubmed: 29167442
Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):349-54
pubmed: 24344307
Nat Methods. 2019 Jul;16(7):633-639
pubmed: 31235883
Nat Commun. 2017 May 25;8:15459
pubmed: 28541304
ACS Synth Biol. 2016 Jan 15;5(1):81-8
pubmed: 26390083
Nucleic Acids Res. 2019 Jan 10;47(1):509-520
pubmed: 30476163
Nat Commun. 2018 Jun 27;9(1):2489
pubmed: 29950558
PLoS One. 2012;7(9):e44852
pubmed: 22984575
Nat Biotechnol. 2013 Sep;31(9):833-8
pubmed: 23907171
Cell. 2013 Sep 12;154(6):1380-9
pubmed: 23992846
Nature. 2019 Aug;572(7768):205-210
pubmed: 31341284
Nat Commun. 2018 Feb 9;9(1):597
pubmed: 29426832
Nat Commun. 2017 Nov 20;8(1):1628
pubmed: 29158476
Science. 2020 Sep 25;369(6511):1637-1643
pubmed: 32820060
Cell. 2013 Dec 19;155(7):1479-91
pubmed: 24360272
Nat Methods. 2014 Jul;11(7):723-6
pubmed: 24797424
Nat Biotechnol. 2014 Jun;32(6):569-76
pubmed: 24770325
Science. 2019 May 10;364(6440):593-597
pubmed: 31000590
Nat Methods. 2015 Dec;12(12):1150-6
pubmed: 26480473
Cell. 2018 Nov 15;175(5):1405-1417.e14
pubmed: 30318144
Sci Rep. 2017 Jul 27;7(1):6732
pubmed: 28751638
Nature. 2019 Aug;572(7768):265-269
pubmed: 31341280
ACS Synth Biol. 2016 Oct 21;5(10):1050-1058
pubmed: 27344932
Cell. 1998 Nov 25;95(5):717-28
pubmed: 9845373
Genome Res. 2014 Jan;24(1):132-41
pubmed: 24253446
Nat Biotechnol. 2014 Jun;32(6):577-582
pubmed: 24770324