The transcriptional elongation rate regulates alternative polyadenylation in yeast.

Poly A / genetics Polyadenylation / genetics RNA Polymerase II / genetics Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae Proteins / genetics Transcription Elongation, Genetic
RNA polymerase II S. cerevisiae chromosomes gene expression gene regulation polyadenylation transcription elongation

Journal

eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614

Informations de publication

Date de publication:
26 08 2020
Historique:
received: 09 06 2020
accepted: 25 08 2020
pubmed: 28 8 2020
medline: 2 3 2021
entrez: 27 8 2020
Statut: epublish

Résumé

Yeast cells undergoing the diauxic response show a striking upstream shift in poly(A) site utilization, with increased use of ORF-proximal poly(A) sites resulting in shorter 3' mRNA isoforms for most genes. This altered poly(A) pattern is extremely similar to that observed in cells containing Pol II derivatives with slow elongation rates. Conversely, cells containing derivatives with fast elongation rates show a subtle downstream shift in poly(A) sites. Polyadenylation patterns of many genes are sensitive to both fast and slow elongation rates, and a global shift of poly(A) utilization is strongly linked to increased purine content of sequences flanking poly(A) sites. Pol II processivity is impaired in diauxic cells, but strains with reduced processivity and normal Pol II elongation rates have normal polyadenylation profiles. Thus, Pol II elongation speed is important for poly(A) site selection and for regulating poly(A) patterns in response to environmental conditions.

Identifiants

DOI: 10.7554/eLife.59810 PMID: 32845240 PMC: PMC7532003
pubmed: 32845240
doi: 10.7554/eLife.59810
pii: 59810
pmc: PMC7532003
doi:
pii:

Substances chimiques

Saccharomyces cerevisiae Proteins 0
Poly A 24937-83-5
RNA Polymerase II EC 2.7.7.-

Banques de données

GEO
['GSE151196']

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM030186
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM131801
Pays : United States
Organisme : NIGMS NIH HHS
ID : R37 GM030186
Pays : United States

Informations de copyright

© 2020, Geisberg et al.

Déclaration de conflit d'intérêts

JG, ZM No competing interests declared, KS Senior editor, eLife

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Auteurs

Joseph V Geisberg (JV)

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, United States.

Zarmik Moqtaderi (Z)

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, United States.
ORCID: 0000-0002-2785-7034

Kevin Struhl (K)

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, United States.
ORCID: 0000-0002-4181-7856

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Polynucléotides › Polyribonucléotides › Poly A › The transcriptional elongation rate regulates...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Maturation post-transcriptionnelle des ARN › Maturation de l'extrémité 3' des ARN › Polyadénylation › The transcriptional elongation rate regulates...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › RNA nucleotidyltransferases › DNA-directed RNA polymerases › RNA polymerase II › The transcriptional elongation rate regulates...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Saccharomyces › Saccharomyces cerevisiae › The transcriptional elongation rate regulates...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines fongiques › Protéines de Saccharomyces cerevisiae › The transcriptional elongation rate regulates...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Transcription génétique › Élongation de la transcription › The transcriptional elongation rate regulates...