Characterization of the Bronchoalveolar Lavage Fluid by Single Cell Gene Expression Analysis in Healthy Dogs: A Promising Technique.
bronchoalveolar lavage fluid
cell
dog
lung
single-cell RNA-sequencing
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2020
2020
Historique:
received:
01
04
2020
accepted:
26
06
2020
entrez:
28
8
2020
pubmed:
28
8
2020
medline:
13
4
2021
Statut:
epublish
Résumé
Single-cell mRNA-sequencing (scRNA-seq) is a technique which enables unbiased, high throughput and high-resolution transcriptomic analysis of the heterogeneity of cells within a population. This recent technique has been described in humans, mice and other species in various conditions to cluster cells in populations and identify new subpopulations, as well as to study the gene expression of cells in various tissues, conditions and origins. In dogs, a species for which markers of cell populations are often limiting, scRNA-seq presents with elevated yet untested potential for the study of tissue composition. As a proof of principle, we used scRNA-seq to identify cellular populations of the bronchoalveolar lavage fluid (BALF) in healthy dogs (
Identifiants
pubmed: 32849601
doi: 10.3389/fimmu.2020.01707
pmc: PMC7406785
doi:
Substances chimiques
RNA, Messenger
0
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1707Informations de copyright
Copyright © 2020 Fastrès, Pirottin, Fievez, Marichal, Desmet, Bureau and Clercx.
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