UDP-glucosyltransferase UGT84B1 regulates the levels of indole-3-acetic acid and phenylacetic acid in Arabidopsis.
Auxin
Indole-3-acetic acid
Metabolism
Phenylacetic acid
UDP-Glucosyltransferase
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
05 11 2020
05 11 2020
Historique:
received:
09
08
2020
accepted:
10
08
2020
pubmed:
2
9
2020
medline:
19
3
2021
entrez:
2
9
2020
Statut:
ppublish
Résumé
Auxin is a key plant growth regulator for diverse developmental processes in plants. Indole-3-acetic acid (IAA) is a primary plant auxin that regulates the formation of various organs. Plants also produce phenylacetic acid (PAA), another natural auxin, which occurs more abundantly than IAA in various plant species. Although it has been demonstrated that the two auxins have distinct transport characteristics, the metabolic pathways and physiological roles of PAA in plants remain unsolved. In this study, we investigated the role of Arabidopsis UDP-glucosyltransferase UGT84B1 in IAA and PAA metabolism. We demonstrated that UGT84B1, which converts IAA to IAA-glucoside (IAA-Glc), can also catalyze the conversion of PAA to PAA-glucoside (PAA-Glc), with a higher catalytic activity in vitro. Furthermore, we showed a significant increase in both the IAA and PAA levels in the ugt84b1 null mutants. However, no obvious developmental phenotypes were observed in the ugt84b1 mutants under laboratory growth conditions. Moreover, the overexpression of UGT84B1 resulted in auxin-deficient root phenotypes and changes in the IAA and PAA levels. Our results indicate that UGT84B1 plays an important role in IAA and PAA homeostasis in Arabidopsis.
Identifiants
pubmed: 32868079
pii: S0006-291X(20)31577-1
doi: 10.1016/j.bbrc.2020.08.026
pmc: PMC7641881
mid: NIHMS1626712
pii:
doi:
Substances chimiques
Arabidopsis Proteins
0
Indoleacetic Acids
0
Phenylacetates
0
indoleacetic acid
6U1S09C61L
Glucosyltransferases
EC 2.4.1.-
UGT85A1 protein, Arabidopsis
EC 2.4.1.-
phenylacetic acid
ER5I1W795A
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
244-250Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM114660
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors have no conflicts of interest to declare.
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