IRX3/5 regulate mitotic chromatid segregation and limb bud shape.

Animals Blotting, Western Chromatids / metabolism Chromosome Segregation / genetics Female Fluorescent Antibody Technique HEK293 Cells Homeodomain Proteins / genetics Humans Immunoprecipitation Limb Buds / embryology Mass Spectrometry Mice Mitosis / genetics Pregnancy RNA-Seq Real-Time Polymerase Chain Reaction Transcription Factors / genetics

Cohesin Iroquois Limb development Mesodermal cell intercalations Mitotic chromatid segregation Morphogenesis

Journal

Development (Cambridge, England)

ISSN: 1477-9129

Titre abrégé: Development

Pays: England

ID NLM: 8701744

Informations de publication

Date de publication:
05 10 2020

Historique:

received: 30 04 2019

accepted: 25 08 2020

pubmed: 11 9 2020

medline: 10 4 2021

entrez: 10 9 2020

Statut: epublish

Résumé

Pattern formation is influenced by transcriptional regulation as well as by morphogenetic mechanisms that shape organ primordia, although factors that link these processes remain under-appreciated. Here we show that, apart from their established transcriptional roles in pattern formation, IRX3/5 help to shape the limb bud primordium by promoting the separation and intercalation of dividing mesodermal cells. Surprisingly, IRX3/5 are required for appropriate cell cycle progression and chromatid segregation during mitosis, possibly in a nontranscriptional manner. IRX3/5 associate with, promote the abundance of, and share overlapping functions with co-regulators of cell division such as the cohesin subunits SMC1, SMC3, NIPBL and CUX1. The findings imply that IRX3/5 coordinate early limb bud morphogenesis with skeletal pattern formation.

Identifiants

DOI: 10.1242/dev.180042 PMID: 32907847 PMC: PMC7561487

pubmed: 32907847

pii: dev.180042

doi: 10.1242/dev.180042

pmc: PMC7561487

pii:

doi:

Substances chimiques

Homeodomain Proteins 0

Irx3 protein, mouse 0

Irx5 protein, mouse 0

Transcription Factors 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

Subventions

Organisme : NIAMS NIH HHS

ID : R01 AR064195

Pays : United States

Organisme : NHLBI NIH HHS

ID : R01 HL148599

Pays : United States

Organisme : CIHR

ID : 137092

Pays : Canada

Informations de copyright

Déclaration de conflit d'intérêts

Competing interestsThe authors declare no competing or financial interests.

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