A whole-genome sequenced control population in northern Sweden reveals subregional genetic differences.


Journal

PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081

Informations de publication

Date de publication:
2020
Historique:
received: 02 03 2020
accepted: 31 07 2020
entrez: 11 9 2020
pubmed: 12 9 2020
medline: 22 10 2020
Statut: epublish

Résumé

The number of national reference populations that are whole-genome sequenced are rapidly increasing. Partly driving this development is the fact that genetic disease studies benefit from knowing the genetic variation typical for the geographical area of interest. A whole-genome sequenced Swedish national reference population (n = 1000) has been recently published but with few samples from northern Sweden. In the present study we have whole-genome sequenced a control population (n = 300) (ACpop) from Västerbotten County, a sparsely populated region in northern Sweden previously shown to be genetically different from southern Sweden. The aggregated variant frequencies within ACpop are publicly available (DOI 10.17044/NBIS/G000005) to function as a basic resource in clinical genetics and for genetic studies. Our analysis of ACpop, representing approximately 0.11% of the population in Västerbotten, indicates the presence of a genetic substructure within the county. Furthermore, a demographic analysis showed that the population from which samples were drawn was to a large extent geographically stationary, a finding that was corroborated in the genetic analysis down to the level of municipalities. Including ACpop in the reference population when imputing unknown variants in a Västerbotten cohort resulted in a strong increase in the number of high-confidence imputed variants (up to 81% for variants with minor allele frequency < 5%). ACpop was initially designed for cancer disease studies, but the genetic structure within the cohort will be of general interest for all genetic disease studies in northern Sweden.

Identifiants

pubmed: 32915809
doi: 10.1371/journal.pone.0237721
pii: PONE-D-20-06062
pmc: PMC7485808
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0237721

Déclaration de conflit d'intérêts

The authors have declared that no competing interests exist.

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Auteurs

Daniel Svensson (D)

Department of Chemistry, Computational Life Science Cluster, Umeå University, Umeå, Sweden.

Matilda Rentoft (M)

Department of Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden.

Anna M Dahlin (AM)

Department of Radiation Sciences, Oncology, Umeå University, Umeå, Sweden.

Emma Lundholm (E)

Centre for Demography and Ageing, Umeå University, Umeå, Sweden.

Pall I Olason (PI)

Science for Life Laboratory, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.

Andreas Sjödin (A)

Department of Chemistry, Computational Life Science Cluster, Umeå University, Umeå, Sweden.
Division of CBRN Security and Defence, FOI-Swedish Defence Research Agency, Umeå, Sweden.

Carin Nylander (C)

Department of Radiation Sciences, Oncology, Umeå University, Umeå, Sweden.

Beatrice S Melin (BS)

Department of Radiation Sciences, Oncology, Umeå University, Umeå, Sweden.

Johan Trygg (J)

Department of Chemistry, Computational Life Science Cluster, Umeå University, Umeå, Sweden.

Erik Johansson (E)

Department of Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden.

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