Convergent and distinctive functions of transcription factors VdYap1, VdAtf1, and VdSkn7 in the regulation of nitrosative stress resistance, microsclerotia formation, and virulence in Verticillium dahliae.
Anacardiaceae
/ microbiology
Ascomycota
/ genetics
Fungal Proteins
/ genetics
Gene Expression Profiling
Gene Expression Regulation, Fungal
Melanins
/ metabolism
Mycelium
Nitric Oxide
/ administration & dosage
Nitrosative Stress
Plant Diseases
/ microbiology
Reactive Nitrogen Species
/ metabolism
Reactive Oxygen Species
/ metabolism
Seedlings
/ microbiology
Sequence Deletion
Transcription Factors
/ genetics
Virulence
Verticillium dahliae
VdAtf1
VdSkn7
VdYap1
nitrosative stress
virulence
Journal
Molecular plant pathology
ISSN: 1364-3703
Titre abrégé: Mol Plant Pathol
Pays: England
ID NLM: 100954969
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
01
03
2020
revised:
09
08
2020
accepted:
14
08
2020
pubmed:
22
9
2020
medline:
29
4
2021
entrez:
21
9
2020
Statut:
ppublish
Résumé
Reactive oxygen/nitrogen species (ROS/RNS) play a fundamental role in plant-fungal interactions. How pathogenic fungi manipulate plant-derived ROS/RNS is of importance to the outcomes of these interactions. In this study, we explored the individual and combined contributions of three transcription factors, VdAtf1, VdYap1, and VdSkn7, in the response to ROS/RNS, microsclerotia formation, and virulence in the plant wilt pathogen Verticillium dahliae. We showed that VdYap1 is essential for ROS response. Additionally, mutants lacking any combination of the three genes shared significant hypersensitivity to nitro-oxidative stress like sodium nitroprusside dehydrate and double deletions lacking VdYap1 and VdAtf1 resulted in further increased sensitivity to ROS. Double deletion of VdAtf1 and VdSkn7 reduced melanin production and virulence while simultaneous lack of VdSkn7 and VdYap1 disrupted nitrogen metabolism and ROS resistance. Finally, comparison of transcriptional profiles of the respective single or double mutants in response to nitro-oxidative stress revealed that the three transcription factors are involved in denitrification of nitrated alkanes and lipids to protect against nitro-oxidative stress. Taken together, our results demonstrate convergent and distinctive functions of VdYap1, VdAtf1, and VdSkn7 in V. dahliae, and provide new data on their roles in response to ROS/RNS in fungi.
Identifiants
pubmed: 32954659
doi: 10.1111/mpp.12988
pmc: PMC7549003
doi:
Substances chimiques
Fungal Proteins
0
Melanins
0
Reactive Nitrogen Species
0
Reactive Oxygen Species
0
Transcription Factors
0
Nitric Oxide
31C4KY9ESH
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1451-1466Informations de copyright
© 2020 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.
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