Posttranslational insertion of small membrane proteins by the bacterial signal recognition particle.
Amino Acid Sequence
Escherichia coli
/ metabolism
Escherichia coli Proteins
/ chemistry
Membrane Proteins
/ metabolism
Models, Biological
Protein Binding
Protein Biosynthesis
Protein Processing, Post-Translational
Protein Transport
RNA, Messenger
/ genetics
SEC Translocation Channels
/ metabolism
Signal Recognition Particle
/ metabolism
Journal
PLoS biology
ISSN: 1545-7885
Titre abrégé: PLoS Biol
Pays: United States
ID NLM: 101183755
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
05
05
2020
accepted:
02
09
2020
revised:
12
10
2020
pubmed:
1
10
2020
medline:
15
12
2020
entrez:
30
9
2020
Statut:
epublish
Résumé
Small membrane proteins represent a largely unexplored yet abundant class of proteins in pro- and eukaryotes. They essentially consist of a single transmembrane domain and are associated with stress response mechanisms in bacteria. How these proteins are inserted into the bacterial membrane is unknown. Our study revealed that in Escherichia coli, the 27-amino-acid-long model protein YohP is recognized by the signal recognition particle (SRP), as indicated by in vivo and in vitro site-directed cross-linking. Cross-links to SRP were also observed for a second small membrane protein, the 33-amino-acid-long YkgR. However, in contrast to the canonical cotranslational recognition by SRP, SRP was found to bind to YohP posttranslationally. In vitro protein transport assays in the presence of a SecY inhibitor and proteoliposome studies demonstrated that SRP and its receptor FtsY are essential for the posttranslational membrane insertion of YohP by either the SecYEG translocon or by the YidC insertase. Furthermore, our data showed that the yohP mRNA localized preferentially and translation-independently to the bacterial membrane in vivo. In summary, our data revealed that YohP engages an unique SRP-dependent posttranslational insertion pathway that is likely preceded by an mRNA targeting step. This further highlights the enormous plasticity of bacterial protein transport machineries.
Identifiants
pubmed: 32997663
doi: 10.1371/journal.pbio.3000874
pii: PBIOLOGY-D-20-01278
pmc: PMC7549839
doi:
Substances chimiques
Escherichia coli Proteins
0
Membrane Proteins
0
RNA, Messenger
0
SEC Translocation Channels
0
Signal Recognition Particle
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3000874Subventions
Organisme : Wellcome Trust
ID : 204957/Z/16/Z
Pays : United Kingdom
Organisme : European Research Council
ID : 769065
Pays : International
Organisme : NIGMS NIH HHS
ID : R15 GM116032
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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