Structural Insights into the Roles of Metazoan-Specific Splicing Factors in the Human Step 1 Spliceosome.
Animals
Catalysis
HeLa Cells
Humans
Introns
/ genetics
Models, Molecular
Multiprotein Complexes
/ metabolism
Protein Binding
Protein Stability
RNA
/ chemistry
RNA Splicing Factors
/ chemistry
Ribonucleoproteins
/ metabolism
Saccharomyces cerevisiae
/ metabolism
Species Specificity
Spliceosomes
/ metabolism
Time Factors
C complex
cryo-EM
human spliceosome
metazoan-specific splicing factors
pre-mRNA splicing
spliceosome structure
step 1 spliceosome
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
01 10 2020
01 10 2020
Historique:
received:
08
05
2020
revised:
08
09
2020
accepted:
09
09
2020
entrez:
2
10
2020
pubmed:
3
10
2020
medline:
21
10
2020
Statut:
ppublish
Résumé
Human spliceosomes contain numerous proteins absent in yeast, whose functions remain largely unknown. Here we report a 3D cryo-EM structure of the human spliceosomal C complex at 3.4 Å core resolution and 4.5-5.7 Å at its periphery, and aided by protein crosslinking we determine its molecular architecture. Our structure provides additional insights into the spliceosome's architecture between the catalytic steps of splicing, and how proteins aid formation of the spliceosome's catalytically active RNP (ribonucleoprotein) conformation. It reveals the spatial organization of the metazoan-specific proteins PPWD1, WDR70, FRG1, and CIR1 in human C complexes, indicating they stabilize functionally important protein domains and RNA structures rearranged/repositioned during the B
Identifiants
pubmed: 33007253
pii: S1097-2765(20)30620-1
doi: 10.1016/j.molcel.2020.09.012
pii:
doi:
Substances chimiques
Multiprotein Complexes
0
RNA Splicing Factors
0
Ribonucleoproteins
0
RNA
63231-63-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
127-139.e6Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing financial interests.