High pressures increase α-chymotrypsin enzyme activity under perchlorate stress.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
02 10 2020
02 10 2020
Historique:
received:
15
05
2020
accepted:
10
09
2020
entrez:
3
10
2020
pubmed:
4
10
2020
medline:
22
6
2021
Statut:
epublish
Résumé
Deep subsurface environments can harbour high concentrations of dissolved ions, yet we know little about how this shapes the conditions for life. We know even less about how the combined effects of high pressure influence the way in which ions constrain the possibilities for life. One such ion is perchlorate, which is found in extreme environments on Earth and pervasively on Mars. We investigated the interactions of high pressure and high perchlorate concentrations on enzymatic activity. We demonstrate that high pressures increase α-chymotrypsin enzyme activity even in the presence of high perchlorate concentrations. Perchlorate salts were shown to shift the folded α-chymotrypsin phase space to lower temperatures and pressures. The results presented here may suggest that high pressures increase the habitability of environments under perchlorate stress. Therefore, deep subsurface environments that combine these stressors, potentially including the subsurface of Mars, may be more habitable than previously thought.
Identifiants
pubmed: 33009512
doi: 10.1038/s42003-020-01279-4
pii: 10.1038/s42003-020-01279-4
pmc: PMC7532203
doi:
Substances chimiques
Perchlorates
0
Chymotrypsin
EC 3.4.21.1
alpha-chymotrypsin
EC 3.4.21.1
perchlorate
VLA4NZX2P4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
550Références
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