Combining metabolic engineering and evolutionary adaptation in Klebsiella oxytoca KMS004 to significantly improve optically pure D-(-)-lactic acid yield and specific productivity in low nutrient medium.
Cassava
D-(−)-lactate
Evolutionary adaptation
K. oxytoca
Metabolic engineering
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
16
03
2020
accepted:
23
09
2020
revised:
15
08
2020
pubmed:
4
10
2020
medline:
8
5
2021
entrez:
3
10
2020
Statut:
ppublish
Résumé
In this study, K. oxytoca KMS004 (ΔadhE Δpta-ackA) was further reengineered by the deletion of frdABCD and pflB genes to divert carbon flux through D-(-)-lactate production. During fermentation of high glucose concentration, the resulted strain named K. oxytoca KIS004 showed poor in growth and glucose consumption due to its insufficient capacity to generate acetyl-CoA for biosynthesis. Evolutionary adaptation was thus employed with the strain to overcome impaired growth and acetate auxotroph. The evolved K. oxytoca KIS004-91T strain exhibited significantly higher glucose-utilizing rate and D-(-)-lactate production as a primary route to regenerate NAD
Identifiants
pubmed: 33009939
doi: 10.1007/s00253-020-10933-0
pii: 10.1007/s00253-020-10933-0
doi:
Substances chimiques
Culture Media
0
Lactic Acid
33X04XA5AT
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
9565-9579Subventions
Organisme : Suranaree University of Technology
ID : OROG-2559