Structural role of essential light chains in the apicomplexan glideosome.
Amino Acid Sequence
Apicomplexa
/ metabolism
Calcium
/ chemistry
Conserved Sequence
Magnetic Resonance Spectroscopy
Models, Molecular
Multiprotein Complexes
/ chemistry
Myosin Light Chains
/ chemistry
Nonmuscle Myosin Type IIA
/ chemistry
Protein Binding
Protein Conformation
Protein Multimerization
Protein Stability
Protozoan Proteins
/ chemistry
Structure-Activity Relationship
Thermodynamics
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
13 10 2020
13 10 2020
Historique:
received:
23
12
2019
accepted:
11
09
2020
entrez:
14
10
2020
pubmed:
15
10
2020
medline:
22
6
2021
Statut:
epublish
Résumé
Gliding, a type of motility based on an actin-myosin motor, is specific to apicomplexan parasites. Myosin A binds two light chains which further interact with glideosome associated proteins and assemble into the glideosome. The role of individual glideosome proteins is unclear due to the lack of structures of larger glideosome assemblies. Here, we investigate the role of essential light chains (ELCs) in Toxoplasma gondii and Plasmodium falciparum and present their crystal structures as part of trimeric sub-complexes. We show that although ELCs bind a conserved MyoA sequence, P. falciparum ELC adopts a distinct structure in the free and MyoA-bound state. We suggest that ELCs enhance MyoA performance by inducing secondary structure in MyoA and thus stiffen its lever arm. Structural and biophysical analysis reveals that calcium binding has no influence on the structure of ELCs. Our work represents a further step towards understanding the mechanism of gliding in Apicomplexa.
Identifiants
pubmed: 33051581
doi: 10.1038/s42003-020-01283-8
pii: 10.1038/s42003-020-01283-8
pmc: PMC7555893
doi:
Substances chimiques
Multiprotein Complexes
0
Myosin Light Chains
0
Protozoan Proteins
0
Nonmuscle Myosin Type IIA
EC 3.6.1.-
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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