Topoisomerase IB interacts with genome segregation proteins and is involved in multipartite genome maintenance in Deinococcus radiodurans.
Bacterial Proteins
/ genetics
Cell Division
Chromosome Segregation
DNA Gyrase
DNA Topoisomerases, Type I
/ genetics
DNA, Bacterial
/ genetics
Deinococcus
/ genetics
Drug Resistance, Bacterial
Escherichia coli
/ genetics
G-Quadruplexes
Gamma Rays
Gene Expression Regulation, Bacterial
/ radiation effects
Genes, Bacterial
Genome, Bacterial
Promoter Regions, Genetic
Radiation Tolerance
Bacterial cell division
Genome segregation
Guanine quadruplexes
Radioresistance
Topoisomerases
Journal
Microbiological research
ISSN: 1618-0623
Titre abrégé: Microbiol Res
Pays: Germany
ID NLM: 9437794
Informations de publication
Date de publication:
Jan 2021
Jan 2021
Historique:
received:
02
05
2020
revised:
18
09
2020
accepted:
19
09
2020
pubmed:
16
10
2020
medline:
8
7
2021
entrez:
15
10
2020
Statut:
ppublish
Résumé
Deinococcus radiodurans, an extremophile, resistant to many abiotic stresses including ionizing radiation, has 2 type I topoisomerases (drTopo IA and drTopo IB) and one type II topoisomerase (DNA gyrase). The role of drTopo IB in guanine quadruplex DNA (G4 DNA) metabolism was demonstrated earlier in vitro. Here, we report that D. radiodurans cells lacking drTopo IB (ΔtopoIB) show sensitivity to G4 DNA binding drug (NMM) under normal growth conditions. The activity of G4 motif containing promoters like mutL and recQ was reduced in the presence of NMM in mutant cells. In mutant, the percentage of anucleate cells was more while the copy number of genome elements were less as compared to wild type. Protein-protein interaction studies showed that drTopo IB interacts with genome segregation and DNA replication initiation (DnaA) proteins. The typical patterns of cellular localization of GFP-PprA were affected in the mutant cells. Microscopic examination of D. radiodurans cells expressing drTopo IB-RFP showed its localization on nucleoid forming a streak parallel to the old division septum and perpendicular to newly formed septum. These results together suggest the role of drTopo IB in genome maintenance in this bacterium.
Identifiants
pubmed: 33059113
pii: S0944-5013(20)30477-8
doi: 10.1016/j.micres.2020.126609
pii:
doi:
Substances chimiques
Bacterial Proteins
0
DNA, Bacterial
0
DNA Topoisomerases, Type I
EC 5.99.1.2
DNA Gyrase
EC 5.99.1.3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
126609Informations de copyright
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