Overexpression of CcNAC1 gene promotes early flowering and enhances drought tolerance of jute (Corchorus capsularis L.).
Drought stress
Jute (Corchorus capsularis L.)
Molecular mechanism
NAC transcription factor
Yeast-2-hybrid
Journal
Protoplasma
ISSN: 1615-6102
Titre abrégé: Protoplasma
Pays: Austria
ID NLM: 9806853
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
19
06
2020
accepted:
12
10
2020
pubmed:
21
10
2020
medline:
6
10
2021
entrez:
20
10
2020
Statut:
ppublish
Résumé
Drought is the main factor that significantly affects plant growth and has devastating effects on crop production of jute. NAC (NAM, ATAF, and CUC2) transcription factors (TFs) are a large gene family in plants that have been shown to play many important roles in regulating developmental processes and abiotic stress resistance. In this study, a NAC transcription factor, CcNAC1, was cloned and characterized its function in jute. RT-qPCR analysis showed that CcNAC1 expression peaks after 8 h of drought stress. CcNAC1 overexpression and knockdown plants were created by Agrobacterium-mediated genetic transformation. PCR and southern hybridization results indicate that the CcNAC1 gene was integrated into the genome of jute. Overexpression of the CcNAC1 gene sped up the plant growth, promoted early flowering, and increased drought tolerance compared to the control plants. 3-Ketoacyl-CoA synthase (KCS) gene expression level increased significantly in the CcNAC1-overexpression plants and decreased in knockdown plants, which showed that CcNAC1 transcription factor regulated KCS gene expression. Yeast-2-Hybrid (Y2H) assays validated the physical interaction between CcNAC1 and KCS. The results provide relatively comprehensive information on the molecular mechanisms of CcNAC1 gene underlying the regulation of plant growth and drought stress resistance, and indicate that CcNAC1 acts as a positive regulator in drought tolerance in jute (Corchorus capsularis L.).
Identifiants
pubmed: 33079225
doi: 10.1007/s00709-020-01569-y
pii: 10.1007/s00709-020-01569-y
doi:
Substances chimiques
Plant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
337-345Subventions
Organisme : National Natural Science Foundation of China
ID : 31860396
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