Adaptive Evolution in Producing Microtiter Cultivations Generates Genetically Stable Escherichia coli Production Hosts for Continuous Bioprocessing.
E. coli
Fab fragments
adaptive evolution
challenging proteins
chemostat cultivation
genetic stability
long-term stability
Journal
Biotechnology journal
ISSN: 1860-7314
Titre abrégé: Biotechnol J
Pays: Germany
ID NLM: 101265833
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
10
08
2020
revised:
06
10
2020
pubmed:
22
10
2020
medline:
28
4
2021
entrez:
21
10
2020
Statut:
ppublish
Résumé
The production of recombinant proteins usually reduces cell fitness and the growth rate of producing cells. The growth disadvantage favors faster-growing non-producer mutants. Therefore, continuous bioprocessing is hardly feasible in Escherichia coli due to the high escape rate. The stability of E. coli expression systems under long-term production conditions and how metabolic load triggered by recombinant gene expression influences the characteristics of mutations are investigated. Iterated fed-batch-like microbioreactor cultivations are conducted under production conditions. The easy-to-produce green fluorescent protein (GFP) and a challenging antigen-binding fragment (Fab) are used as model proteins, and BL21(DE3) and BL21
Identifiants
pubmed: 33084246
doi: 10.1002/biot.202000376
doi:
Substances chimiques
Recombinant Proteins
0
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2000376Informations de copyright
© 2020 The Authors. Biotechnology Journal published by Wiley-VCH GmbH.
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