Rab-E and its interaction with myosin XI are essential for polarised cell growth.
Arabidopsis thaliana
Physcomitrella patens
Rab-E GTPase
myosin XI
polarised growth
vesicle trafficking
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
02
05
2020
accepted:
12
10
2020
pubmed:
25
10
2020
medline:
15
5
2021
entrez:
24
10
2020
Statut:
ppublish
Résumé
The fundamental process of polarised exocytosis requires the interconnected activity of molecular motors trafficking vesicular cargo within a dynamic cytoskeletal network. In plants, few mechanistic details are known about how molecular motors, such as myosin XI, associate with their secretory cargo to support the ubiquitous processes of polarised growth and cell division. Live-cell imaging coupled with targeted gene knockouts and a high-throughput RNAi assay enabled the first characterisation of the loss of Rab-E function. Yeast two-hybrid and subsequent in silico structural prediction uncovered a specific interaction between Rab-E and myosin XI that is conserved between P. patens and A. thaliana. Rab-E co-localises with myosin XI at sites of active exocytosis, and at the growing tip both proteins are spatiotemporally coupled. Rab-E is required for normal plant growth in P. patens and the rab-E and myosin XI phenotypes are rescued by A. thaliana's Rab-E1c and myosin XI-K/E, respectively. Both PpMyoXI and AtMyoXI-K interact with PpRabE14, and the interaction is specifically mediated by PpMyoXI residue V1422. This interaction is required for polarised growth. Our results suggest that the interaction of Rab-E and myosin XI is a conserved feature of polarised growth in plants.
Identifiants
pubmed: 33098085
doi: 10.1111/nph.17023
pmc: PMC8168425
mid: NIHMS1640813
doi:
Substances chimiques
Plant Proteins
0
Myosins
EC 3.6.4.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1924-1936Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM068803
Pays : United States
Organisme : NIGMS NIH HHS
ID : R15 GM134493
Pays : United States
Informations de copyright
© 2020 The Authors New Phytologist © 2020 New Phytologist Foundation.
Références
Small GTPases. 2018 Mar 4;9(1-2):22-48
pubmed: 28632484
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Plant Physiol. 2009 Apr;149(4):1824-37
pubmed: 19233904
Plant Cell. 2013 Aug;25(8):3022-38
pubmed: 23995081
Plant J. 2016 Nov;88(4):531-541
pubmed: 27420177
Plant J. 2019 Oct;100(2):279-297
pubmed: 31264742
Small GTPases. 2019 Mar;10(2):111-121
pubmed: 28394692
Elife. 2014 Sep 23;3:
pubmed: 25247701
Plant Physiol. 2008 Mar;146(3):1109-16
pubmed: 18178669
Annu Rev Plant Biol. 2013;64:243-65
pubmed: 23451782
Genome Biol. 2016 Jul 05;17(1):148
pubmed: 27380939
Plant Cell. 2010 Sep;22(9):3053-65
pubmed: 20870962
Nat Protoc. 2011 Aug 11;6(9):1341-54
pubmed: 21886100
Methods Mol Biol. 2019;1992:307-322
pubmed: 31148047
J Cell Sci. 2020 Feb 26;133(4):
pubmed: 31964706
Dev Cell. 2011 Dec 13;21(6):1156-70
pubmed: 22172676
J Mol Biol. 2000 Dec 8;304(4):585-98
pubmed: 11099382
Plant Physiol. 2018 Jan;176(1):119-127
pubmed: 29162634
J Vis Exp. 2011 Apr 19;(50):
pubmed: 21540817
Plant J. 2013 Feb;73(3):417-28
pubmed: 23020796
Plant Physiol. 2008 Aug;147(4):1646-58
pubmed: 18508956
Genetics. 2012 Jun;191(2):347-87
pubmed: 22701052
Planta. 2013 Jan;237(1):161-72
pubmed: 23001196
Traffic. 2009 Oct;10(10):1377-89
pubmed: 19522756
Dev Cell. 2012 Oct 16;23(4):769-81
pubmed: 23079598
Plant Physiol. 2018 Jan;176(1):352-363
pubmed: 28972078
Nucleic Acids Res. 2014 Jul;42(Web Server issue):W285-9
pubmed: 24829450
Biochem Biophys Res Commun. 2018 Nov 25;506(2):409-421
pubmed: 29339158
Curr Opin Plant Biol. 2002 Dec;5(6):518-28
pubmed: 12393015
J Exp Bot. 2009;60(1):197-212
pubmed: 19039101
Annu Rev Cell Dev Biol. 2001;17:159-87
pubmed: 11687487
Microbiol Mol Biol Rev. 2018 Apr 11;82(2):
pubmed: 29643171
Front Plant Sci. 2015 Oct 19;6:878
pubmed: 26539200
Dev Cell. 2014 Jun 9;29(5):607-620
pubmed: 24882377
Plant Cell. 2005 Jul;17(7):2020-36
pubmed: 15972698
Curr Opin Cell Biol. 2020 Feb;62:46-53
pubmed: 31546159
Plant Cell. 2008 Jan;20(1):101-23
pubmed: 18239134
Trends Biotechnol. 2019 Jul;37(7):761-774
pubmed: 30654913
Plant Cell. 2000 Nov;12(11):2201-18
pubmed: 11090219
J Cell Biol. 1999 Nov 15;147(4):791-808
pubmed: 10562281
J Exp Bot. 2018 May 25;69(12):2869-2881
pubmed: 29579267
Plant Biotechnol J. 2017 Jan;15(1):122-131
pubmed: 27368642
Nat Commun. 2018 Dec 14;9(1):5313
pubmed: 30552321
Plant Cell. 2009 Oct;21(10):3026-40
pubmed: 19861555
Plant Cell. 2017 Oct;29(10):2610-2625
pubmed: 28970336
Nucleic Acids Res. 2018 Jul 2;46(W1):W296-W303
pubmed: 29788355
J Cell Sci. 2009 Dec 1;122(Pt 23):4383-92
pubmed: 19903693
EMBO J. 2006 Feb 22;25(4):693-700
pubmed: 16437158
Plant Cell. 2010 Jun;22(6):1868-82
pubmed: 20525854
PLoS One. 2013 Oct 07;8(10):e76745
pubmed: 24116145
Cell Microbiol. 2007 Jan;9(1):1-8
pubmed: 17081192
Plant Biol (Stuttg). 2005 May;7(3):251-7
pubmed: 15912444
Mol Biol Cell. 2008 Oct;19(10):4177-87
pubmed: 18653471
Traffic. 2012 Jul;13(7):898-907
pubmed: 22420621
Annu Rev Biochem. 2012;81:637-59
pubmed: 22463690
Mol Biol Cell. 2017 Mar 15;28(6):712-715
pubmed: 28292916
J Cell Sci. 2020 Feb 26;133(4):
pubmed: 32102937
Curr Opin Plant Biol. 2013 Dec;16(6):710-7
pubmed: 24446546
Proc Natl Acad Sci U S A. 2010 Apr 6;107(14):6346-51
pubmed: 20308558
J Integr Plant Biol. 2015 Jan;57(1):79-92
pubmed: 25431342
Plant Cell. 2007 Nov;19(11):3705-22
pubmed: 17981997
Structure. 2013 Aug 6;21(8):1284-97
pubmed: 23931141
Front Plant Sci. 2012 Sep 03;3:184
pubmed: 22969781
Plant Physiol. 2003 Oct;133(2):470-4
pubmed: 14555775
Curr Opin Plant Biol. 2008 Dec;11(6):610-9
pubmed: 18952493
Plant Physiol. 2020 Oct;184(2):607-619
pubmed: 32764132
BMC Plant Biol. 2012 May 17;12:70
pubmed: 22594499