Genetic engineering of potato (Solanum tuberosum L.) for enhanced α-tocopherols and abiotic stress tolerance.
Journal
Physiologia plantarum
ISSN: 1399-3054
Titre abrégé: Physiol Plant
Pays: Denmark
ID NLM: 1256322
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
revised:
14
10
2020
received:
18
07
2020
accepted:
23
10
2020
pubmed:
26
10
2020
medline:
21
8
2021
entrez:
25
10
2020
Statut:
ppublish
Résumé
Vitamin E (α-tocopherol) is a lipid-soluble essential vitamin recognized for improvement in degenerative health conditions, abating cancer risk, and coronary heart diseases in humans. While in plants, it acts as a free radical scavenger that protects cells against oxidative and photooxidative damages. The daily consumption of potato makes it a key target for biofortification with vitamins for eliminating vitamin deficiency in large populations. Vitamin E biosynthetic pathway genes have been overexpressed in plants via genetic engineering to enhance the α-tocopherol content. Major genes involved in the vitamin E biosynthesis in plants viz. the homogentisate-phytyltransferase (At-HPT) and γ-tocopherol-methyltransferase (At-γ-TMT), isolated from Arabidopsis were constitutively overexpressed in potato (Solanum tuberosum L.). The molecular analyses of independent transgenic lines revealed a stable integration of both the genes in the plant genome. The transgenic potato exhibited significantly improved vitamin E contents up to 173-258% in comparison to the untransformed control plants. Transgenic tissues also exhibited increased cellular antioxidant enzymes, proline, osmolyte, and glutathione content that are directly correlated with the ability of the plant to withstand abiotic stresses imposed by salt (NaCl) and heavy metal (CdCl
Substances chimiques
alpha-Tocopherol
H4N855PNZ1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
116-128Subventions
Organisme : Department of Science and Technology, New Delhi, INDIA
ID : DST/WOS-A/ 2013/LS-420
Informations de copyright
© 2020 Scandinavian Plant Physiology Society.
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