Priming Is Dispensable for NLRP3 Inflammasome Activation in Human Monocytes
Caspase 1
/ metabolism
Humans
Inflammasomes
/ metabolism
Interleukin-18
/ metabolism
Interleukin-1beta
/ metabolism
Intracellular Signaling Peptides and Proteins
/ metabolism
Macrophage Activation
Macrophages
/ immunology
Monocytes
/ immunology
NLR Family, Pyrin Domain-Containing 3 Protein
/ metabolism
Nigericin
/ pharmacology
Phosphate-Binding Proteins
/ metabolism
Protein Multimerization
Pyroptosis
Signal Transduction
THP-1 Cells
GSDMD
IL-18
NLRP3
inflammasome
macrophage
monocytes
priming
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2020
2020
Historique:
received:
26
05
2020
accepted:
14
09
2020
entrez:
26
10
2020
pubmed:
27
10
2020
medline:
4
6
2021
Statut:
epublish
Résumé
Interleukin (IL)-18 and IL-1β are potent pro-inflammatory cytokines that contribute to inflammatory conditions such as rheumatoid arthritis and Alzheimer's disease. They are produced as inactive precursors that are activated by large macromolecular complexes called inflammasomes upon sensing damage or pathogenic signals. NLRP3 inflammasome activation is regarded to require a priming step that causes NLRP3 and IL-1β gene upregulation, and also NLRP3 post-translational licencing. A subsequent activation step leads to the assembly of the complex and the cleavage of pro-IL-18 and pro-IL-1β by caspase-1 into their mature forms, allowing their release. Here we show that human monocytes, but not monocyte derived macrophages, are able to form canonical NLRP3 inflammasomes in the absence of priming. NLRP3 activator nigericin caused the processing and release of constitutively expressed IL-18 in an unprimed setting. This was mediated by the canonical NLRP3 inflammasome that was dependent on K
Identifiants
pubmed: 33101286
doi: 10.3389/fimmu.2020.565924
pmc: PMC7555430
doi:
Substances chimiques
GSDMD protein, human
0
Inflammasomes
0
Interleukin-18
0
Interleukin-1beta
0
Intracellular Signaling Peptides and Proteins
0
NLR Family, Pyrin Domain-Containing 3 Protein
0
Phosphate-Binding Proteins
0
Caspase 1
EC 3.4.22.36
Nigericin
RRU6GY95IS
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
565924Subventions
Organisme : Medical Research Council
ID : MR/N029992/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/N013751/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 110091
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 104192/Z/14/Z
Pays : United Kingdom
Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2020 Gritsenko, Yu, Martin-Sanchez, Diaz-del-Olmo, Nichols, Davis, Brough and Lopez-Castejon.
Références
J Clin Invest. 2013 Nov;123(11):4695-705
pubmed: 24084736
J Pharmacol Exp Ther. 1998 Feb;284(2):687-92
pubmed: 9454815
J Clin Invest. 2016 Nov 1;126(11):4388
pubmed: 27801679
Cell Death Dis. 2019 Feb 25;10(3):190
pubmed: 30804327
J Biol Chem. 2018 Aug 10;293(32):12563-12575
pubmed: 29929983
J Biol Chem. 2007 Dec 14;282(50):36321-9
pubmed: 17959595
EMBO Rep. 2018 Oct;19(10):
pubmed: 30206189
Trends Neurosci. 2005 Sep;28(9):487-93
pubmed: 16023742
Am J Physiol Cell Physiol. 2016 Jul 1;311(1):C83-C100
pubmed: 27170638
Curr Opin Immunol. 2011 Oct;23(5):591-7
pubmed: 21839623
Proc Natl Acad Sci U S A. 2018 Oct 2;115(40):E9371-E9380
pubmed: 30232264
Cell Rep. 2018 Nov 6;25(6):1525-1536.e7
pubmed: 30404007
J Immunol. 2001 Sep 1;167(5):2879-86
pubmed: 11509635
J Immunol. 2000 Oct 15;165(8):4615-23
pubmed: 11035104
J Leukoc Biol. 2016 May;99(5):723-34
pubmed: 26574023
J Biol Chem. 2012 Oct 19;287(43):36617-22
pubmed: 22948162
Cell Death Differ. 2016 Jul;23(7):1219-31
pubmed: 26868913
Cell Chem Biol. 2017 Nov 16;24(11):1321-1335.e5
pubmed: 28943355
Brain Res Mol Brain Res. 1995 Jul;31(1-2):122-30
pubmed: 7476020
J Leukoc Biol. 1994 Nov;56(5):542-7
pubmed: 7964161
J Exp Med. 2018 May 7;215(5):1315-1325
pubmed: 29549113
Nat Immunol. 2016 Oct;17(10):1176-86
pubmed: 27548431
Cell Death Dis. 2012 Jul 05;3:e338
pubmed: 22764097
FEBS Lett. 2004 Jul 30;571(1-3):50-4
pubmed: 15280016
Immunity. 2013 Jun 27;38(6):1142-53
pubmed: 23809161
Front Immunol. 2020 Sep 30;11:565924
pubmed: 33101286
Cell Death Differ. 2003 Sep;10(9):956-68
pubmed: 12934070
Trends Biochem Sci. 2016 Dec;41(12):1012-1021
pubmed: 27669650
Cell Res. 2010 Dec;20(12):1289-305
pubmed: 20856264
Mol Cell. 2013 Jan 24;49(2):331-8
pubmed: 23246432
Int J Biochem Cell Biol. 2010 May;42(5):585-9
pubmed: 20060931
Proc Natl Acad Sci U S A. 2014 Jan 14;111(2):775-80
pubmed: 24379360
J Biol Chem. 2014 Nov 21;289(47):32926-36
pubmed: 25288801
Immunity. 2012 Sep 21;37(3):487-500
pubmed: 22981536
Nat Cell Biol. 2020 Jun;22(6):716-727
pubmed: 32367047
J Biol Chem. 2019 May 24;294(21):8325-8335
pubmed: 30940725
Inflamm Res. 2018 Oct;67(10):879-889
pubmed: 30136196
Mol Cell Proteomics. 2010 May;9(5):880-93
pubmed: 20173201
Trends Mol Med. 2017 Feb;23(2):165-180
pubmed: 28109721
Nat Chem Biol. 2019 Jun;15(6):560-564
pubmed: 31086329
Cell Res. 2015 Dec;25(12):1285-98
pubmed: 26611636
J Immunol Methods. 2017 Aug;447:1-13
pubmed: 28268194
J Allergy Clin Immunol. 2017 May;139(5):1698-1701
pubmed: 27876626
Nat Immunol. 2014 Aug;15(8):738-48
pubmed: 24952504
Methods Mol Biol. 2013;1040:91-101
pubmed: 23852599
J Exp Med. 2017 Jun 5;214(6):1725-1736
pubmed: 28465465
Cell Rep. 2017 Dec 19;21(12):3414-3426
pubmed: 29262323
Am J Hum Genet. 2002 Jul;71(1):198-203
pubmed: 12032915
Cell Rep. 2017 Jan 31;18(5):1285-1297
pubmed: 28147281
J Biol Chem. 2001 Feb 9;276(6):3820-6
pubmed: 11056157
J Immunol. 2009 Jul 15;183(2):787-91
pubmed: 19570822
J Biol Chem. 2008 Nov 28;283(48):33080-6
pubmed: 18829460
Nature. 2015 Oct 29;526(7575):660-5
pubmed: 26375003
Nat Rev Immunol. 2013 Jun;13(6):397-411
pubmed: 23702978
Nature. 2016 Jul 7;535(7610):111-6
pubmed: 27281216
Brain Res Rev. 2009 Apr;60(1):57-64
pubmed: 19166877
J Exp Med. 2017 Sep 4;214(9):2671-2693
pubmed: 28716882
J Invest Dermatol. 2018 Jun;138(6):1380-1390
pubmed: 29287762
Mol Cell. 2017 Oct 5;68(1):185-197.e6
pubmed: 28943315
Oncotarget. 2015 Mar 20;6(8):5490-500
pubmed: 25823927
Cytokine. 2018 Apr;104:143-146
pubmed: 29017770
Sci Rep. 2019 Mar 14;9(1):4563
pubmed: 30872589
Nat Rev Immunol. 2016 Jul;16(7):407-20
pubmed: 27291964
Signal Transduct Target Ther. 2017;2:
pubmed: 29158945
Immunity. 2016 Apr 19;44(4):833-46
pubmed: 27037191
PLoS One. 2017 Apr 12;12(4):e0175336
pubmed: 28403163