A novel biosensor for the detection of organophosphorus (OP)-based pesticides using organophosphorus acid anhydrolase (OPAA)-FL variant.
Acetylcholinesterase (AChE)
Organophosphorus acid anhydrolase (OPAA)-FL variant
Organophosphorus compounds (OPs)
Paraoxon
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Jan 2021
Jan 2021
Historique:
received:
11
03
2020
accepted:
08
11
2020
revised:
28
10
2020
pubmed:
17
11
2020
medline:
15
5
2021
entrez:
16
11
2020
Statut:
ppublish
Résumé
Indiscriminate use of organophosphorus (OP)-based insecticides is a great concern to human health because of bioaccumulation-induced health hazards. Potentially fatal consequences and limited treatment methods of OP poisoning necessitate the need for the development of reliable, selective, cost-effective, and sensitive methods of OP detection. To tackle this issue, the development of effective devices and methods is required to sensitively detect as well as degrade OPs. Enzymatic sensor systems have gained popularity due to high catalytic activity, enhanced detection limits, and high sensitivity with the environmentally benign operation. Organophosphorus acid anhydrolase (OPAA) from Alteromonas sp. JD6.5 is capable of hydrolyzing the P-F, P-O, P-S, and P-CN bonds, in OPs, including nerve agents of the G/V-series. Several mutants of OPAA are reported which have greater activity against various OPs. In this study, recombinant expression of the OPAA-FL variant in Escherichia coli was performed, purified, and subsequently tested for activity against ethyl paraoxon. OPAA-FL variant showed its optimum activity at pH 8.5 and 50 °C. Colorimetric and fluorometric assays were used for estimation of ethyl paraoxon based on p-nitrophenol and fluorescein isothiocyanate (FITC) fluorescence intensity, respectively. Colorimetric and fluorometric assay estimation indicates that ethyl paraoxon can be estimated in the linear range of 0.01 to 1 mM and 0.1 to 0.5 mM, with LOD values 0.04 mM and 0.056 mM, respectively. Furthermore, the OPAA-FL variant was immobilized into alginate microspheres for colorimetric detection of ethyl paraoxon and displayed a linear range of 0.025 to 1 mM with a LOD value of 0.06 mM. KEY POINTS: • Biosensing of paraoxon with purified and encapsulated OPAA-FL variant. • Colorimetric and fluorometric biosensing assay developed using OPAA-FL variant for paraoxon. • First report on alginate encapsulation of OPAA-FL variant for biosensing of paraoxon. Graphical abstract.
Identifiants
pubmed: 33191461
doi: 10.1007/s00253-020-11008-w
pii: 10.1007/s00253-020-11008-w
doi:
Substances chimiques
Organophosphorus Compounds
0
Pesticides
0
Aryldialkylphosphatase
EC 3.1.8.1
Paraoxon
Q9CX8P80JW
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
389-400Subventions
Organisme : Department of Biotechnology, Govt. of India
ID : BT/PR20319/BBE/117/189/2016
Organisme : Department of Biotechnology, Govt. of India
ID : BT/PR24652/NER/95/795/2017
Organisme : DST-INSPIRE
ID : IF170325
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