Structure of the O-Antigen and the Lipid A from the Lipopolysaccharide of Fusobacterium nucleatum ATCC 51191.
Fusobacterium nucleatum
Gram-negative bacteria
MALDI-TOF mass spectrometry
NMR spectroscopy
O-antigens
lipid A
lipopolysaccharides (LPS)
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
06 04 2021
06 04 2021
Historique:
revised:
14
11
2020
received:
31
10
2020
pubmed:
17
11
2020
medline:
18
11
2021
entrez:
16
11
2020
Statut:
ppublish
Résumé
Fusobacterium nucleatum is a common member of the oral microbiota. However, this symbiont has been found to play an active role in disease development. As a Gram-negative bacterium, F. nucleatum has a protective outer membrane layer whose external leaflet is mainly composed of lipopolysaccharides (LPSs). LPSs play a crucial role in the interaction between bacteria and the host immune system. Here, we characterised the structure of the O-antigen and lipid A from F. nucleatum ssp. animalis ATCC 51191 by using a combination of GC-MS, MALDI and NMR techniques. The results revealed a novel repeat of the O-antigen structure of the LPS, [→4)-β-d-GlcpNAcA-(1→4)-β-d-GlcpNAc3NAlaA-(1→3)-α-d-FucpNAc4NR-(1→], (R=acetylated 60 %), and a bis-phosphorylated hexa-acylated lipid A moiety. Taken together these data showed that F. nucleatum ATCC 51191 has a distinct LPS which might differentially influence recognition by immune cells.
Identifiants
pubmed: 33197108
doi: 10.1002/cbic.202000751
pmc: PMC8048906
doi:
Substances chimiques
Lipid A
0
Lipopolysaccharides
0
O Antigens
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1252-1260Subventions
Organisme : UniNA
Organisme : Compagnia di San Paolo
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/F/00044452
Pays : United Kingdom
Organisme : BBSRC Institute Strategic Programme Grant Gut Microbes and Health
ID : BB/R012490/1
Informations de copyright
© 2020 The Authors. ChemBioChem published by Wiley-VCH GmbH.
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