Molecular basis for cysteine oxidation by plant cysteine oxidases from Arabidopsis thaliana.

Amino Acid Sequence Arabidopsis / metabolism Arabidopsis Proteins / metabolism Arginine / metabolism Cysteine / metabolism Cysteine Dioxygenase / metabolism Oxidation-Reduction
Cysteine oxidation Mass spectrometry Plant Cysteine Oxidase(s) X-ray crystallography

Journal

Journal of structural biology
ISSN: 1095-8657
Titre abrégé: J Struct Biol
Pays: United States
ID NLM: 9011206

Informations de publication

Date de publication:
03 2021
Historique:
received: 14 10 2020
revised: 09 11 2020
accepted: 10 11 2020
pubmed: 19 11 2020
medline: 15 12 2021
entrez: 18 11 2020
Statut: ppublish

Résumé

Plant Cysteine Oxidases (PCOs) play important roles in controlling the stability of Group VII ethylene response factors (ERF-VIIs) via Arg/N-degron pathway through catalyzing the oxidation of their N-Cys for subsequent Arginyl-tRNA--protein transferase 1 (ATE1) mediated arginine installation. Here we presented the crystal structures of PCO2, PCO4, and PCO5 from Arabidopsis thaliana (AtPCOs) and examined their in vitro activity by Mass spectrometry (MS). On the basis of Tris-bound AtPCO2, we modelled the structure of Cys-bound AtPCO2 and identified key AtPCO2 residues involved in N-Cys recognition and oxidation. Alanine substitution of potential N-Cys interaction residues impaired the activity of AtPCO5 remarkably. The structural research, complemented by mutagenesis and MS experiments, not only uncovers the substrate recognition and catalytic mode by AtPCOs, but also sheds light on the future design of potent inhibitors for plant cysteine oxidases.

Identifiants

DOI: 10.1016/j.jsb.2020.107663 PMID: 33207269
pubmed: 33207269
pii: S1047-8477(20)30236-7
doi: 10.1016/j.jsb.2020.107663
pii:
doi:

Substances chimiques

Arabidopsis Proteins 0
Arginine 94ZLA3W45F
Cysteine Dioxygenase EC 1.13.11.20
Cysteine K848JZ4886

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

107663

Informations de copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

Auteurs

Zhenzhen Chen (Z)

The First Affiliated Hospital of University of Science and Technology of China (Anhui Provincial Hospital), 230000 Hefei, China; MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.

Qiong Guo (Q)

MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.

Gao Wu (G)

The First Affiliated Hospital of University of Science and Technology of China (Anhui Provincial Hospital), 230000 Hefei, China.

Jie Wen (J)

The First Affiliated Hospital of University of Science and Technology of China (Anhui Provincial Hospital), 230000 Hefei, China. Electronic address: jiewen@ustc.edu.cn.

Shanhui Liao (S)

MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China. Electronic address: ajsod@mail.ustc.edu.cn.

Chao Xu (C)

MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China. Electronic address: xuchaor@ustc.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Molecular basis for cysteine oxidation by...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Brassicaceae › Arabidopsis › Molecular basis for cysteine oxidation by...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Protéines d'Arabidopsis › Molecular basis for cysteine oxidation by...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés essentiels › Arginine › Molecular basis for cysteine oxidation by...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés soufrés › Cystéine › Molecular basis for cysteine oxidation by...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Oxygénases › Dioxygenases › Cysteine dioxygenase › Molecular basis for cysteine oxidation by...
questionsmedicales.fr › Métabolisme › Métabolisme énergétique › Oxydoréduction › Molecular basis for cysteine oxidation by...