An efficient Oligo-FISH painting system for revealing chromosome rearrangements and polyploidization in Triticeae.
Aegilops
/ genetics
Chromosome Painting
/ methods
Chromosomes, Plant
/ genetics
Gene Library
Gene Rearrangement
/ genetics
Genetic Linkage
/ genetics
Hordeum
/ genetics
In Situ Hybridization, Fluorescence
/ methods
Oligonucleotides
/ genetics
Poaceae
/ genetics
Secale
/ genetics
Translocation, Genetic
/ genetics
Triticum
/ genetics
Oligo-FISH
Triticeae species
chromosome rearrangement
wheat-barley synteny
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
03
09
2020
revised:
25
10
2020
accepted:
09
11
2020
pubmed:
20
11
2020
medline:
29
6
2021
entrez:
19
11
2020
Statut:
ppublish
Résumé
A chromosome-specific painting technique has been developed which combines the most recent approaches of the companion disciplines of molecular cytogenetics and genome research. We developed seven oligonucleotide (oligo) pools derivd from single-copy sequences on chromosomes 1 to 7 of barley (Hordeum vulgare L.) and corresponding collinear regions of wheat (Triticum aestivum L.). The seven groups of pooled oligos comprised between 10 986 and 12 496 45-bp monomers, and these then produced stable fluorescence in situ hybridization (FISH) signals on chromosomes of each linkage group of wheat and barley. The pooled oligo probes were applied to high-throughput karyotyping of the chromosomes of other Triticeae species in the genera Secale, Aegilops, Thinopyrum, and Dasypyrum, and the study also extended to some wheat-alien amphiploids and derived lines. We demonstrated that a complete set of whole-chromosome oligo painting probes facilitated the study of inter-species chromosome homologous relationships and visualized non-homologous chromosomal rearrangements in Triticeae species and some wheat-alien species derivatives. When combined with other non-denaturing FISH procedures using tandem-repeat oligos, the newly developed oligo painting techniques provide an efficient tool for the study of chromosome structure, organization, and evolution among any wild Triticeae species with non-sequenced genomes.
Substances chimiques
Oligonucleotides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
978-993Informations de copyright
© 2020 Society for Experimental Biology and John Wiley & Sons Ltd.
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