Investigating Mitochondrial Transcriptomes and RNA Processing Using Circular RNA Sequencing.
Animals
Computational Biology
/ methods
Gene Expression Profiling
/ methods
Genome, Mitochondrial
High-Throughput Nucleotide Sequencing
/ methods
Humans
Mice
Mitochondria
/ genetics
RNA Processing, Post-Transcriptional
/ genetics
RNA, Circular
/ genetics
RNA, Mitochondrial
/ genetics
Rats
Sequence Analysis, RNA
/ methods
Software
Transcriptome
Mitochondria
Next generation sequencing
RNA processing
RNA-Seq
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
24
11
2020
pubmed:
25
11
2020
medline:
30
3
2021
Statut:
ppublish
Résumé
Transcriptomic technologies have revolutionized the study of gene expression and RNA biology. Different RNA sequencing methods enable the analyses of diverse species of transcripts, including their abundance, processing, stability, and other specific features. Mitochondrial transcriptomics has benefited from these technologies that have revealed the surprising complexity of its RNAs. Here we describe a method based upon cyclization of mitochondrial RNAs and next generation sequencing to analyze the steady-state levels and sizes of mitochondrial RNAs, their degradation products, as well as their processing intermediates by capturing both 5' and 3' ends of transcripts.
Identifiants
pubmed: 33230764
doi: 10.1007/978-1-0716-0834-0_4
doi:
Substances chimiques
RNA, Circular
0
RNA, Mitochondrial
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM