Kinetic/thermodynamic study of immobilized β-fructofuranosidase from Aspergillus tamarii URM4634 in chitosan beads and application on invert sugar production in packed bed reactor.

Aspergillus Chitosan Fructose Glucose Thermodynamics beta-Fructofuranosidase
Aspergillus Enzymatic reactor Immobilization Invert sugar Kinetic Thermodynamic β-fructofuranosidases

Journal

Food research international (Ottawa, Ont.)
ISSN: 1873-7145
Titre abrégé: Food Res Int
Pays: Canada
ID NLM: 9210143

Informations de publication

Date de publication:
11 2020
Historique:
received: 31 03 2020
revised: 13 09 2020
accepted: 13 09 2020
entrez: 25 11 2020
pubmed: 26 11 2020
medline: 15 5 2021
Statut: ppublish

Résumé

β-fructofuranosidase (FFase) from Aspergillus tamarii URM4634 was immobilized covalently in chitosan beads. It was characterized biochemically, studied in terms of kinetic and thermodynamic parameters, and applied on conversion of sucrose for invert sugar production in a packed bed reactor (PBR). The optimum reactional conditions were determined and obtained at pH 5.0 and 60 °C. FFase was thermostable at 50-55°C. At 50°C, the enzyme shows longer half-life (t

Identifiants

DOI: 10.1016/j.foodres.2020.109730 PMID: 33233298
pubmed: 33233298
pii: S0963-9969(20)30755-9
doi: 10.1016/j.foodres.2020.109730
pii:
doi:

Substances chimiques

Fructose 30237-26-4
Chitosan 9012-76-4
beta-Fructofuranosidase EC 3.2.1.26
invert sugar ED959S6ACY
Glucose IY9XDZ35W2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

109730

Informations de copyright

Copyright © 2020 Elsevier Ltd. All rights reserved.

Auteurs

Rodrigo Lira de Oliveira (RL)

Northeast Biotechnology Network/RENORBIO, Federal Rural University of Pernambuco (UFRPE), Dom Manoel de Medeiros, Dois Irmãos, 52171-900 Recife, PE, Brazil.

Vinícius Luís Vilela Dos Santos (VLV)

Academic Unit of Garanhuns/UAG, Federal Rural University of Pernambuco (UFRPE), Av. Bom Pastor, Boa Vista, 55296-901 Garanhuns, PE, Brazil.

Marcos Fellipe da Silva (MF)

Academic Unit of Garanhuns/UAG, Federal Rural University of Pernambuco (UFRPE), Av. Bom Pastor, Boa Vista, 55296-901 Garanhuns, PE, Brazil; Bioprocess and Metabolic Engineering Laboratory, Department Food Engineering, University of Campinas (UNICAMP), Cidade Universitária Zeferino Vaz, 80th Monteiro Lobato, 13.083-862, Campinas, São Paulo, Brazil.

Tatiana Souza Porto (TS)

Northeast Biotechnology Network/RENORBIO, Federal Rural University of Pernambuco (UFRPE), Dom Manoel de Medeiros, Dois Irmãos, 52171-900 Recife, PE, Brazil; Academic Unit of Garanhuns/UAG, Federal Rural University of Pernambuco (UFRPE), Av. Bom Pastor, Boa Vista, 55296-901 Garanhuns, PE, Brazil. Electronic address: portots@yahoo.com.br.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Champignons › Deuteromycota › Aspergillus › Kinetic/thermodynamic study of immobilized...
questionsmedicales.fr › Glucides › Polyosides › Chitine › Chitosane › Kinetic/thermodynamic study of immobilized...
questionsmedicales.fr › Glucides › Sucres › Oses › Cétoses (sucres) › Fructose › Kinetic/thermodynamic study of immobilized...
questionsmedicales.fr › Glucides › Sucres › Oses › Hexose › Glucose › Kinetic/thermodynamic study of immobilized...
questionsmedicales.fr › Phénomènes physiques › Thermodynamique › Kinetic/thermodynamic study of immobilized...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › beta-Fructofuranosidase › Kinetic/thermodynamic study of immobilized...