Carbon Isotopes
Coronavirus Nucleocapsid Proteins
/ chemistry
Crystallography, X-Ray
Dimerization
Drug Design
Hydrogen
Hydrogen-Ion Concentration
Magnetic Resonance Spectroscopy
Nitrogen Isotopes
Phosphoproteins
/ chemistry
Protein Binding
Protein Domains
Protein Interaction Mapping
Protein Structure, Secondary
SARS-CoV-2
/ chemistry
Covid19-NMR
Dimerization domain
Nucleocapsid
Protein druggability
SARS-CoV-2
Solution NMR-spectroscopy
Structural protein
Journal
Biomolecular NMR assignments
ISSN: 1874-270X
Titre abrégé: Biomol NMR Assign
Pays: Netherlands
ID NLM: 101472371
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
received:
24
10
2020
accepted:
25
11
2020
pubmed:
4
12
2020
medline:
7
4
2021
entrez:
3
12
2020
Statut:
ppublish
Résumé
The current outbreak of the highly infectious COVID-19 respiratory disease is caused by the novel coronavirus SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2). To fight the pandemic, the search for promising viral drug targets has become a cross-border common goal of the international biomedical research community. Within the international Covid19-NMR consortium, scientists support drug development against SARS-CoV-2 by providing publicly available NMR data on viral proteins and RNAs. The coronavirus nucleocapsid protein (N protein) is an RNA-binding protein involved in viral transcription and replication. Its primary function is the packaging of the viral RNA genome. The highly conserved architecture of the coronavirus N protein consists of an N-terminal RNA-binding domain (NTD), followed by an intrinsically disordered Serine/Arginine (SR)-rich linker and a C-terminal dimerization domain (CTD). Besides its involvement in oligomerization, the CTD of the N protein (N-CTD) is also able to bind to nucleic acids by itself, independent of the NTD. Here, we report the near-complete NMR backbone chemical shift assignments of the SARS-CoV-2 N-CTD to provide the basis for downstream applications, in particular site-resolved drug binding studies.
Identifiants
pubmed: 33270159
doi: 10.1007/s12104-020-09995-y
pii: 10.1007/s12104-020-09995-y
pmc: PMC7711055
doi:
Substances chimiques
Carbon Isotopes
0
Coronavirus Nucleocapsid Proteins
0
Nitrogen Isotopes
0
Nitrogen-15
0
Phosphoproteins
0
nucleocapsid phosphoprotein, SARS-CoV-2
0
Hydrogen
7YNJ3PO35Z
Carbon-13
FDJ0A8596D
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
129-135Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : SFB902/B18
Organisme : Deutsche Forschungsgemeinschaft
ID : SCHL2062/2-1
Organisme : JQYA
ID : 2019/AS01
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