Quantification of Cerebral Perfusion using Laser Speckle Imaging and Infarct Volume using MRI in a Pre-clinical Model of Posterior Circulation Stroke.
Animals
Arterial Occlusive Diseases
/ complications
Basilar Artery
/ diagnostic imaging
Cerebral Infarction
/ diagnosis
Computed Tomography Angiography
Diffusion Magnetic Resonance Imaging
Disease Models, Animal
Dogs
Lasers
Magnetic Resonance Angiography
Magnetic Resonance Imaging
Perfusion
Stroke
/ diagnosis
Stroke Volume
Journal
Journal of visualized experiments : JoVE
ISSN: 1940-087X
Titre abrégé: J Vis Exp
Pays: United States
ID NLM: 101313252
Informations de publication
Date de publication:
17 11 2020
17 11 2020
Historique:
entrez:
7
12
2020
pubmed:
8
12
2020
medline:
29
12
2020
Statut:
epublish
Résumé
Basilar artery occlusion (BAO) is a subset of posterior circulation stroke that carries a mortality as high as 90%. The current clinical standard to diagnose ischemic stroke include computerized tomography (CT), CT angiography and perfusion and magnetic resonance imaging (MRI). Large animal pre-clinical models to accurately reflect the clinical disease as well as methods to assess stroke burden and evaluate treatments are lacking. We describe a canine model of large vessel occlusion (LVO) stroke in the posterior circulation, and developed a laser speckle imaging (LSI) protocol to monitor perfusion changes in real time. We then utilized high b-value DWI (b=1800s/mm Our studies demonstrate real time perfusion imaging using LSI of a canine thromboembolic LVO model of posterior circulation stroke, which utilizes multimodal imaging important in the diagnosis and treatment of ischemic stroke.
Sections du résumé
BACKGROUND
Basilar artery occlusion (BAO) is a subset of posterior circulation stroke that carries a mortality as high as 90%. The current clinical standard to diagnose ischemic stroke include computerized tomography (CT), CT angiography and perfusion and magnetic resonance imaging (MRI). Large animal pre-clinical models to accurately reflect the clinical disease as well as methods to assess stroke burden and evaluate treatments are lacking.
METHODS
We describe a canine model of large vessel occlusion (LVO) stroke in the posterior circulation, and developed a laser speckle imaging (LSI) protocol to monitor perfusion changes in real time. We then utilized high b-value DWI (b=1800s/mm
CONCLUSIONS
Our studies demonstrate real time perfusion imaging using LSI of a canine thromboembolic LVO model of posterior circulation stroke, which utilizes multimodal imaging important in the diagnosis and treatment of ischemic stroke.
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Video-Audio Media
Langues
eng
Sous-ensembles de citation
IM