Investigation of the role of AcTPR2 in kiwifruit and its response to Botrytis cinerea infection.
Abscisic Acid
/ metabolism
Actinidia
/ genetics
Botrytis
/ genetics
Catalase
/ metabolism
Disease Resistance
/ genetics
Fruit
/ genetics
Gene Expression Regulation, Fungal
Gene Expression Regulation, Plant
Host-Pathogen Interactions
/ genetics
Indoleacetic Acids
/ metabolism
Peroxidase
/ metabolism
Phenylalanine Ammonia-Lyase
/ metabolism
Plant Diseases
/ genetics
Plant Growth Regulators
/ metabolism
Plant Proteins
/ genetics
Plants, Genetically Modified
Superoxide Dismutase
/ metabolism
AcTPR2
Botrytis cinerea
IAA signaling
Kiwifruit
Virus-induced gene silencing
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
10 Dec 2020
10 Dec 2020
Historique:
received:
22
07
2020
accepted:
02
12
2020
entrez:
11
12
2020
pubmed:
12
12
2020
medline:
14
9
2021
Statut:
epublish
Résumé
Elucidation of the regulatory mechanism of kiwifruit response to gray mold disease caused by Botrytis cinerea can provide the basis for its molecular breeding to impart resistance against this disease. In this study, 'Hongyang' kiwifruit served as the experimental material; the TOPLESS/TOPLESS-RELATED (TPL/TPR) co-repressor gene AcTPR2 was cloned into a pTRV2 vector (AcTPR2-TRV) and the virus-induced gene silencing technique was used to establish the functions of the AcTPR2 gene in kiwifruit resistance to Botrytis cinerea. Virus-induced silencing of AcTPR2 enhanced the susceptibility of kiwifruit to Botrytis cinerea. Defensive enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and phenylalanine ammonia-lyase (PAL) and endogenous phytohormones such as indole acetic acid (IAA), gibberellin (GA The results of the present study could help clarify the regulatory mechanisms of disease resistance in kiwifruit and furnish genetic resources for molecular breeding of kiwifruit disease resistance.
Sections du résumé
BACKGROUND
BACKGROUND
Elucidation of the regulatory mechanism of kiwifruit response to gray mold disease caused by Botrytis cinerea can provide the basis for its molecular breeding to impart resistance against this disease. In this study, 'Hongyang' kiwifruit served as the experimental material; the TOPLESS/TOPLESS-RELATED (TPL/TPR) co-repressor gene AcTPR2 was cloned into a pTRV2 vector (AcTPR2-TRV) and the virus-induced gene silencing technique was used to establish the functions of the AcTPR2 gene in kiwifruit resistance to Botrytis cinerea.
RESULTS
RESULTS
Virus-induced silencing of AcTPR2 enhanced the susceptibility of kiwifruit to Botrytis cinerea. Defensive enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and phenylalanine ammonia-lyase (PAL) and endogenous phytohormones such as indole acetic acid (IAA), gibberellin (GA
CONCLUSIONS
CONCLUSIONS
The results of the present study could help clarify the regulatory mechanisms of disease resistance in kiwifruit and furnish genetic resources for molecular breeding of kiwifruit disease resistance.
Identifiants
pubmed: 33302873
doi: 10.1186/s12870-020-02773-x
pii: 10.1186/s12870-020-02773-x
pmc: PMC7731759
doi:
Substances chimiques
Indoleacetic Acids
0
Plant Growth Regulators
0
Plant Proteins
0
indoleacetic acid
6U1S09C61L
Abscisic Acid
72S9A8J5GW
Catalase
EC 1.11.1.6
Peroxidase
EC 1.11.1.7
Superoxide Dismutase
EC 1.15.1.1
Phenylalanine Ammonia-Lyase
EC 4.3.1.24
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
557Subventions
Organisme : National Natural Science Foundation of China
ID : 32001351
Organisme : National Natural Science Foundation of China
ID : 31670688
Organisme : Natural Science Foundation of Chongqing
ID : cstc2018jscx-msybX0196
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